Indian Journal of Medical Microbiology (Indian J Med Microbiol)

Publications in this journal

• Article: Isolation and characterization of the first vancomycin dependent Enterococcus from India

  Tuhina Banerjee, Shampa Anupurba
  Indian Journal of Medical Microbiology 03/2013; 31(1):91-92.
• Article: Evaluation of antigenicity and cell mediated immunity of Hepatitis E virus patients: Using non radioactive MTT assay

  Majumdar M, Ratho R, Chawla Y, Singh MP
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  ABSTRACT: Hepatitis E virus (HEV) is an important cause of hepatitis in developing nations. Disease spans from asymptomatic infection to acute viral hepatitis (AVH) and acute liver failure (ALF). Cell-mediated immunity (CMI) is less studied. Studies document CMI in HEV patients using [ 3 H]-thymidine incorporation (radioactive in nature). The aim of this study was to evaluate the antigenicity of recombinant HEV ORF 2 peptide (452-617 a.a) (pORF2) by non-radioactive MTT assay and detecting the proliferation indices of primary PBMC culture. A total of 27 laboratory confirmed HEV patients (16 AVH and 11 ALF) and 20 apparently healthy individuals (HC) were included. PBMCs were isolated, plated and stimulated with pORF2. After an incubation of 4 days, cells were looked for blastogenic transformation and subjected to MTT assay. PI of AVH, ALF and healthy controls were found to be 3.249 ± 0.219, 1.748 ± 0.076 and 0.226 ± 0.017, respectively. PI of AVH Vs HC, ALF Vs HC and AVH Vs ALF were found to be significantly higher ( P < 0.0001). This study demonstrates MTT to be an adaptable technique to evaluate CMI in HEV patients. Recombinant pORF2 was found to be antigenic in nature and PBMCs from AVH patients were immunologically more reactive than ALF patients.
  Indian Journal of Medical Microbiology 01/2013; 31(1):64-8.
• Article: Artemisia annua leaves and seeds mediate programmed cell death in Leishmania donovani promastigotes

  • Islamuddin M, Farooque A, Dwarakanath BS, Sahal D, Afrin F
  Indian Journal of Medical Microbiology 01/2012;
• Article: Comparison of primers for the detection of genogroup II noroviruses in India

  S George, VK Menon, S Ramani, G Kang
  Indian Journal of Medical Microbiology 01/2012;
• Article: Prolonged jaundice attributed to super infection of hepatitis E virus in a case of resolving leptospirosis

  Singh MP, Majumdar M, Sharma A, Chawla Y, Ratho RK
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  ABSTRACT: India is endemic for both Leptospira and hepatitis E virus (HEV). The clinical presentations of these diseases have overlapping features. We report a case of superinfection of HEV in a patient with resolving leptospirosis with underlying Hodgkin lymphoma. The diagnosis of HEV in our case was established by HEV-RNA PCR as our patient was immunosuppressed. The present study highlights the need for molecular diagnosis in the case of HEV infection with strong clinical suspicion and negative serological results.
  Indian Journal of Medical Microbiology 10/2011; 30(1):103-6.
• Article: Kinetics of chemokines secretion in human macrophages infected with various strains of Mycobacterium tuberculosis

  Rajvelu P, SD Das
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  ABSTRACT: Indian J Med Microbiol. 2010, 28(3):201-6.
  Indian Journal of Medical Microbiology 01/2010; 28(3):201-06.
• Article: Fatal haemophagocytic syndrome and hepatitis associated with visceral leishmaniasis.

  P Mathur, J C Samantaray, P Samanta
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  ABSTRACT: Haemophagocytic syndrome (HPS) secondary to infections occurs due to excessive, non-malignant proliferation of histiocytes, with resultant haemophagocytosis. The syndrome is essentially treatable, provided timely etiological diagnosis is achieved. In this report, we present a rare case of a child who hailed from Uttaranchal and presented with severe hepatitis. Bone marrow examination revealed an unexpected diagnosis of HPS secondary to visceral leishmaniasis. Despite initiating appropriate antileishmanial treatment, the child had a fatal outcome.
  Indian Journal of Medical Microbiology 11/2007; 25(4):416-8.
• Article: Immunobiology of human imunodeficiency virus infection.

  P Tripathi, S Agrawal
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  ABSTRACT: After the discovery of human immunodeficiency virus (HIV) and its role in the causation of most devastating epidemic acquired immune deficiency syndrome (AIDS), there has been an increasing trend to decipher the mechanism of infection and to understand why it cannot be controlled by our immune system. By evolution, our immune system has been empowered and enough trained to recognize, elicit immune response and remove antigens and pathogens from the body. Simultaneously, HIV has also gained enough mechanism to escape the natural immune response. On one hand, it downregulates HLA class I antigens, which may present viral antigens to specific CD8 + T cells; on the other hand, the viral genome get mutated very readily under the selection pressure of specific cytotoxic T lymphocytes. The high mutation rate and convertibility of its genotype makes it a moving target and poses a prime hurdle in vaccine development. This review explains how HIV enters into the cell, how it resists the host immune response and how HIV manages to escape from it and establish in the human body.
  Indian Journal of Medical Microbiology 11/2007; 25(4):311-22.