Diseases of Aquatic Organisms (DIS AQUAT ORGAN)

Publisher: Inter Research

Journal description

Diseases affect all facets of life - at the cell, tissue, organ, individual, population and ecosystem level. Since life originated in an aquatic medium, studies of disease phenomena in the wide array of aquatic taxa contribute significantly to the analysis, comprehension, prevention and treatment of diseases in general, including those of organisms now inhabiting terrestrial environments and of humans. DAO aims to fully cover these important research areas

Current impact factor: 1.75

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 1.752
2013 Impact Factor 1.586
2012 Impact Factor 1.734
2011 Impact Factor 2.201
2010 Impact Factor 1.572
2009 Impact Factor 1.687
2008 Impact Factor 1.586
2007 Impact Factor 1.598
2006 Impact Factor 1.509
2005 Impact Factor 1.361
2004 Impact Factor 1.583
2003 Impact Factor 1.263
2002 Impact Factor 1.561
2001 Impact Factor 1.653
2000 Impact Factor 1.556
1999 Impact Factor 1.515
1998 Impact Factor 1.213
1997 Impact Factor 1.183
1996 Impact Factor 1.181
1995 Impact Factor 1.071
1994 Impact Factor 0.906
1993 Impact Factor 0.948
1992 Impact Factor 1.123

Impact factor over time

Impact factor

Additional details

5-year impact 1.99
Cited half-life >10.0
Immediacy index 0.38
Eigenfactor 0.01
Article influence 0.59
Website Diseases of Aquatic Organisms website
Other titles Diseases of aquatic organisms, DAO
ISSN 0177-5103
OCLC 13369805
Material type Periodical, Internet resource
Document type Journal / Magazine / Newspaper, Internet Resource

Publisher details

Inter Research

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Author's pre-print on author's personal website, institutional website or institutional repository
    • Author's post-print on author's personal website or institutional website
    • Author's post-print on institutional repository after 12 months embargo
    • Authors covered by funding agency rules, may post author's post-print in PubMed Central after a 6 months embargo
    • If mandated by a funding agency or institution, the author's post-print may be deposited in institutional repository before 12 months, as long addendum is submitted
    • Publisher's version/PDF may be used after 5 years embargo
    • Publisher copyright and source must be acknowledged
    • Reviewed 31/01/2014
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: The monogenean Dactylogyrus intermedius and the bacterium Flavobacterium columnare are 2 common pathogens in aquaculture. The objective of the present study was to examine the effect of prior parasitism by D. intermedius on the susceptibility of goldfish to F. columnare and to explore the potential immune mechanisms related to the parasite infection. A F. columnare challenge trial was conducted between D. intermedius-parasitized and non-parasitized goldfish. The F. columnare load in gill, kidney, spleen and liver were compared. The expression of immune-related genes (IL-1β2, TNF-α1, TGF-β, iNOS-a, C3 and Lyz) in gill and kidney of D. intermedius-only infected and uninfected control fish were evaluated. D. intermedius-parasitized goldfish exhibited higher mortality and significantly higher loads (3051 to 537379 genome equivalents [GEs] mg-1) of F. columnare, which were 1.13 to 50.82-fold higher than non-parasitized fish (389 to 17829 GEs mg-1). Furthermore, the immune genes IL-1β2, TNF-α1, iNOS-a and Lyz were up-regulated while the TGF-β and C3 were down-regulated in the gill and kidney of parasite-infected fish compared to the non-parasitized controls. The down-regulation TGF-β and C3 was especially noteworthy, as this might indicate the suppression of the host immune functions due to the parasitism by D. intermedius. Taken together, these data demonstrate that parasite infection can enhance bacterial invasion and presents a hypothesis, based on gene expression data, that modulation of host immune response could play a role.
    Diseases of Aquatic Organisms 09/2015; 116(1). DOI:10.3354/dao02902
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    ABSTRACT: A neonate Risso's dolphin Grampus griseus was found stranded alive on a beach in Catalonia, Spain. Rehabilitation attempts were unsuccessful and it died 2 d later, showing pneumonia and sepsis. A pure bacterial culture was obtained from all tissues and blood and identified as Aeromonas hydrophila using the API 20NE. However, sequencing the rpoD gene showed that the strain in fact belongs to A. dhakensis, making this the first report of fatal haemorrhagic-necrotizing pneumonia and sepsis due to this species in a marine mammal. The A. dhakensis strain GMV-704 produced β-haemolysis, possessed several virulence genes and showed sensitivity to several antimicrobials. This study provides a new potential host for A. dhakensis, and its potential virulence in dolphins and its presence in the marine environment may warrant considering this species a potential threat to marine mammals.
    Diseases of Aquatic Organisms 09/2015; 116(1):69-74. DOI:10.3354/dao02899
  • Diseases of Aquatic Organisms 09/2015; 115(1):1-6.
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    ABSTRACT: Soft tunic syndrome in the edible ascidian Halocynthia roretzi is caused by the kinetoplastid flagellate Azumiobodo hoyamushi, which was found to assume a fusiform cell form with 2 flagella in axenic, pure culture. When the flagellate form was incubated in sterilized artificial seawater (pH 8.4), some of the cells became cyst-like and adhered to the bottom of the culture plate. The cyst-like forms were spherical or cuboidal, and each had 2 flagella encapsulated in its cytoplasm. Encystment was also induced in culture medium alkalified to the pH of seawater (8.4) but not in unmodified (pH 7.2) or acidified media (pH 6.4). More than 95% of the cyst-like cells converted to the flagellate form within 1 d following transfer to seawater containing ascidian tunic extracts from host ascidians. The cyst-like cells were able to survive in seawater with no added nutrients for up to 2 wk at 20°C and for a few months at 5 to 15°C. The survival period in seawater depended on temperature: some cyst-like cells survived 3 mo at 10°C, and ca. 95% of these converted to flagellate forms in seawater containing tunic extracts. Thus, A. hoyamushi is able to persist under adverse conditions in a cyst-like form able to adhere to organic and inorganic substrata for protracted periods of time.
    Diseases of Aquatic Organisms 08/2015; 115(3):253-62. DOI:10.3354/dao02897
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    ABSTRACT: Mucocutaneous lesions were biopsied from free-ranging Atlantic bottlenose dolphins Tursiops truncatus inhabiting the Indian River Lagoon (IRL), Florida, and estuarine waters of Charleston (CHS), South Carolina, USA, between 2003 and 2013. A total of 78 incisional biopsies from 58 dolphins (n = 43 IRL, n = 15 CHS) were examined. Thirteen dolphins had 2 lesions biopsied at the same examination, and 6 dolphins were re-examined and re-biopsied at time intervals varying from 1 to 8 yr. Biopsy sites included the skin (n = 47), tongue (n = 2), and genital mucosa (n = 29). Pathologic diagnoses were: orogenital sessile papilloma (39.7%), cutaneous lobomycosis (16.7%), tattoo skin disease (TSD; 15.4%), nonspecific chronic to chronic-active dermatitis (15.4%), and epidermal hyperplasia (12.8%). Pathologic diagnoses from dolphins with 2 lesions were predominately orogenital sessile papillomas (n = 9) with nonspecific chronic to chronic-active dermatitis (n = 4), TSD (n = 3), lobomycosis (n = 1), and epidermal hyperplasia (n = 1). Persistent pathologic diagnoses from the same dolphins re-examined and re-biopsied at different times included genital sessile papillomas (n = 3), lobomycosis (n = 2), and nonspecific dermatitis (n = 2). This is the first study documenting the various types, combined prevalence, and progression of mucocutaneous lesions in dolphins from the southeastern USA. The data support other published findings describing the health patterns in dolphins from these geographic regions. Potential health impacts related to the observed suite of lesions are important for the IRL and CHS dolphin populations, since previous studies have indicated that both populations are affected by complex infectious diseases often associated with immunologic disturbances and anthropogenic contaminants.
    Diseases of Aquatic Organisms 08/2015; 115(3):175-84. DOI:10.3354/dao02895
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    ABSTRACT: The highly virulent fungal pathogen Batrachochytrium dendrobatidis (Bd) poses a global threat to amphibian biodiversity. Streams and other water bodies are central habitats in the ecology of the disease, particularly in rainforests where they may transport and transmit the pathogen and harbor infected tadpoles that serve as reservoir hosts. We conducted an experiment using larval green-eyed tree frogs Litoria serrata in semi-natural streamside channels to test the hypotheses that (1) the fungus can be transmitted downstream in stream habitats and (2) infection affects tadpole growth and mouthpart loss. Our results showed that transmission can occur downstream in flowing water with no contact between individuals, that newly infected tadpoles suffered increased mouthpart loss in comparison with controls that were never infected and that infected tadpoles grew at reduced rates. Although recently infected tadpoles showed substantial loss of mouthparts, individuals with longstanding infections did not, suggesting that mouthparts may re-grow following initial loss. Our study suggests that any management efforts that can reduce the prevalence of infections in tadpoles may be particularly effective if applied in headwater areas, as their effects are likely to be felt downstream.
    Diseases of Aquatic Organisms 08/2015; 115(3):213-21. DOI:10.3354/dao02898
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    ABSTRACT: Herpesviral hematopoietic necrosis caused by goldfish hematopoietic necrosis virus (now identified as cyprinid herpesvirus 2, CyHV-2) has contributed to economic losses in goldfish Carassius auratus culture and is becoming a major obstacle in Prussian carp C. gibelio aquaculture in China. Several reports have described difficulties in culturing the virus, with the total loss of infectivity within several passages in cell culture. We succeeded in propagating CyHV-2 with a high infectious titer in a RyuF-2 cell line newly derived from the fin of the Ryukin goldfish variety using culture medium supplemented with 0.2% healthy goldfish kidney extract. The addition of kidney extract to the medium enabled rapid virus growth, resulting in the completion of cytopathic effect (CPE) within 4 to 6 d at 25°C. The extract also enabled reproducible virus culture with a titer of 105-6 TCID50 ml-1. The virus cultured using this protocol showed pathogenicity in goldfish after intraperitoneal injection. The virus grew in RyuF-2 cells at 15, 20, 25, 30, and 32°C but not at 34°C or higher. Higher incubation temperatures allowed earlier development of CPE, but culture at 30 and 32°C yielded a lower virus titer than that obtained at other temperatures because of heat inactivation of the propagated virus during cultivation. Cell lines derived from goldfish and ginbuna C. langsdorfii showed high susceptibility to the virus; cell lines from carp were susceptible to the virus using a medium containing goldfish kidney extract, but EPC, FHM, and BF-2 cell lines did not produce any CPE, even in the presence of the extract.
    Diseases of Aquatic Organisms 08/2015; 115:223-232. DOI:10.3354/dao02885
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    ABSTRACT: Fibropapillomatosis (FP) comprises a majority of green turtle stranding in Hawaii; however, green turtles in the Pacific are also susceptible to non-FP related causes of death. We present here necropsy findings from 230 free-ranging green turtles originating from Hawaii, the Mariana archipelago, Palmyra Atoll, American Samoa, and Johnston Atoll that died from non-FP related causes. Most turtles died from fishing-induced or boat strike trauma followed by infectious/inflammatory diseases, nutritional problems (mainly cachexia), and an array of physiologic problems. Infectious/inflammatory problems included bacterial diseases of the lungs, eyes, liver or intestines, spirorchid fluke infection, or polyarthritis of unknown origin. Likelihood of a successful diagnosis of cause of death was a function of post-mortem decomposition. Fibropapillomatosis was not seen in turtles submitted from outside Hawaii. The preponderance of anthropogenic causes of mortality offers some management opportunities to mitigate causes of death in these animals by, for example, implementing measures to decrease boating and fishing interactions.
    Diseases of Aquatic Organisms 07/2015; 115(2):103-110. DOI:10.3354/dao02890
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    ABSTRACT: A quantitative PCR (qPCR) assay was developed for Flavobacterium psychrophilum, the causative agent of bacterial coldwater disease. The assay was targeted to fp1493 as it encodes a putative outer membrane protein (FP1493) that is reactive to the monoclonal antibody (MAb FL43) used in a standardized F. psychrophilum capture enzyme-linked immunosorbent assay (ELISA). The qPCR was specific to F. psychrophilum and was able to detect between 8 and 809000 copies of fp1493. To determine if antigen level in the tissue was indicative of bacterial concentration, kidney samples from 108 steelhead Oncorhynchus mykiss and coho salmon O. kisutch female broodstock were screened by ELISA and qPCR. There was no correlation between ELISA optical density (OD) values and the number of F. psychrophilum cells g-1 of kidney tissue as estimated by qPCR (rS = 0.42; p > 0.05). The median number of F. psychrophilum cells in steelhead samples was 6.11 × 103 cells g-1 of tissue. For coho salmon samples, the median number of cells was 3.95 × 103 cells g-1 of tissue. Agreement between the 2 assays was less than 50%. As fp1493 is a single-copy gene and differential expression of FP1493 has been reported, we hypothesize that the discrepancy between the 2 assays is due to increased expression of FP1493 in the in vivo environment. Therefore, ELISA OD values most likely provide an indication of differential protein expression, while the qPCR assay estimates bacterial load in tissue.
    Diseases of Aquatic Organisms 07/2015; 115(2). DOI:10.3354/dao02881