Electrophoresis Journal Impact Factor & Information

Publisher: Electrophoresis Society; International Electrophoresis Society, Wiley-VCH Verlag

Journal description

Attracting high-quality papers from the world's leading laboratories ELECTROPHORESIS has proved itself indispensable in the life sciences where electrophoresis is the most widely used method. ELECTROPHORESIS is the foremost journal for new analytical and preparative methods and for innovative applications on all aspects of electrophoresis. It has an international scope and publishes original papers short communications and review articles in the following areas: biochemistry molecular and cell biochemistry genetics immunology microbiology clinical chemistry forensics food science and many more. Special Issues Paper Symposium issues devoted to new developments prepared by an expert Guest Editor are published regularly with special emphasis on capillary electrophoresis (2-3 issues per year)and proteomics. The journal also publishes proceedings of international and national electrophoresis meetings.

Current impact factor: 3.03

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 3.028
2013 Impact Factor 3.161
2012 Impact Factor 3.261
2011 Impact Factor 3.303
2010 Impact Factor 3.569
2009 Impact Factor 3.077
2008 Impact Factor 3.509
2007 Impact Factor 3.609
2006 Impact Factor 4.101
2005 Impact Factor 3.85
2004 Impact Factor 3.743
2003 Impact Factor 4.04
2002 Impact Factor 4.325
2001 Impact Factor 4.282
2000 Impact Factor 3.385
1999 Impact Factor 3.447
1998 Impact Factor 3.054
1997 Impact Factor 2.848
1996 Impact Factor 2.467
1995 Impact Factor 2.73
1994 Impact Factor 2.274
1993 Impact Factor 1.842
1992 Impact Factor 2.159

Impact factor over time

Impact factor

Additional details

5-year impact 2.72
Cited half-life 7.90
Immediacy index 0.53
Eigenfactor 0.02
Article influence 0.58
Website Electrophoresis website
Other titles Electrophoresis, Proteomics reviews
ISSN 0173-0835
OCLC 7297725
Material type Periodical, Internet resource
Document type Journal / Magazine / Newspaper, Internet Resource

Publisher details

Wiley-VCH Verlag

  • Pre-print
    • Author cannot archive a pre-print version
  • Post-print
    • Author cannot archive a post-print version
  • Restrictions
    • Upon funder agreement with publisher
  • Conditions
    • Pre-print may be deposited on personal intranet or institutional intranet repository, but not on a public repository
    • Pre-print must not updates with future versions
    • Published source must be acknowledged with set phrases (See policy)
    • Must link to publisher's site: http://www.interscience.wiley.com/
    • Publisher's version/PDF cannot be used
    • Some journal exceptions-check individual homepages
  • Classification

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: The performance of capillary electrophoresis (CE) coupled on-line to mass spectrometry (MS) via a sheathless porous tip sprayer was evaluated for anionic metabolic profiling. A representative metabolite mixture and biological samples were used for the evaluation of various analytical parameters, such as peak efficiency (plate numbers), migration time and peak area repeatability, and limits of detection (LODs). The background electrolyte (BGE), i.e. 10% acetic acid (pH 2.2), previously used for cationic metabolic profiling was now assessed for anionic metabolic profiling by using MS detection in negative ion mode. For test compounds, relative standard deviations (RSDs) for migration times and peak areas were below 2% and 11%, respectively, and plate numbers ranged from 60000 to 400000 demonstrating a high separation efficiency. Critical metabolites with low or no retention on reversed-phase LC could be efficiently separated and selectively analysed by the sheathless CE-MS method. An injection volume of only circa 20 nL resulted in LODs between 10 and 200 nM (corresponding to an amount of 0.4 to 4 fmol), which was an at least tenfold improvement as compared to LODs obtained by conventional CE-MS approaches for these analytes. The methodology was applied to anionic metabolic profiling of glioblastoma cell line extracts. Overall, a sheathless CE-MS method has been developed for highly efficient and sensitive anionic metabolic profiling studies, which can also be used for cationic metabolic profiling studies by only switching the MS detection and separation voltage polarity. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500435
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    ABSTRACT: A frequently utilised method of data quantification in western blot analysis is comparison of the protein of interest with a house keeping gene or control protein. Commonly used proteins include β-actin, glyceraldehyde 3 phosphate dehydrogenase (GAPDH), and α-tubulin. Various reliability issues have been raised when using this technique for data analysis - particularly when investigating protein expression changes during development and in disease states. In this study we have demonstrated that β-actin, GAPDH, and α-tubulin are not appropriate controls in the study of development and hypoxic-ischemic induced damage in the piglet brain. We have also shown that using an in-house pooled standard, loaded on all blots is a reliable method for controlling inter-assay variability and data normalisation in protein expression analysis. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500385
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    ABSTRACT: Simultaneous extraction of acidic and basic analytes from a sample is seen to be a challenging task. In this work, a novel and efficient electromembrane extraction (EME) method based on two separate cells was applied to simultaneously extract and preconcentrate two acidic drugs (naproxen and ibuprofen) along with a basic drug (ketamine). Once both cells were filled with the sample solution, basic drug was extracted from one cell with the other cell used to extract acidic drugs. The employed supported liquid membranes (SLMs) for the extraction of acidic and basic drugs were 2-ethyl hexanol and 1-octanol, respectively. Under an applied potential of 250V in the course of the extraction process, acidic and basic drugs were extracted from a 3.0 mL aqueous sample solution into 25 μL acceptor solutions. The pH values of the donor and acceptor solutions in the cathodic cell were 5.0 and 1.5, respectively, the corresponding values in the anodic cell were, however, 8.0 and 12.5, respectively. The rates of recovery obtained within 20 min of extraction time at a stirring rate of 750 rpm ranged from 45 to 54%. With correlation coefficients ranging from 0.990 to 0.996, the proposed EME technique provided good linearity over a concentration range of 20-1000 ng mL(-1) . The limit of detection for all drugs was found to be 6.7 ng mL(-1) , while reproducibility ranged from 7 to 12% (n = 5). Finally, applying the proposed method to determine and quantify the drugs in urine and wastewater samples, satisfactory results were achieved. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500455
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    ABSTRACT: Rapid Electrokinetic Patterning (REP) is an emerging optoelectric technique that takes advantage of laser-induced AC electrothermal flow and particle-electrode interactions to trap and translate particles. The electrothermal flow in REP is driven by the temperature rise induced by the laser absorption in the thin electrode layer. In previous REP applications 350 to 700 nm indium tin oxide (ITO) layers have been used as electrodes. In this study, we show that ITO is an inefficient electrode choice as more than 92% of the irradiated laser on the ITO electrodes is transmitted without absorption. Using theoretical, computational and experimental approaches, we demonstrate that for a given laser power the temperature rise is controlled by both the electrode material and its thickness. A 25 nm thick Ti electrode creates an electrothermal flow of the same speed as a 700 nm thick ITO electrode while requiring only 14% of the laser power used by ITO. These results represent an important step in the design of low-cost portable REP systems by lowering the material cost and power consumption of the system. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500473

  • Electrophoresis 11/2015; DOI:10.1002/elps.201500394

  • Electrophoresis 11/2015; DOI:10.1002/elps.201500436

  • Electrophoresis 09/2015; DOI:10.1002/elps.201500284
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    ABSTRACT: The cellular prion protein (PrPC) is a glycoprotein, anchored to the plasma membrane and abundantly expressed in the central nervous system. The expression of PrPC is low in the peripheral tissues and only little information is available on its functions in the non-neuronal tissues. The antioxidant function of PrPC during the activation of hepatic stellate cells has already been reported. Therefore, the aim of the study was to expand our knowledge on the functions of PrPC by detailed characterization of its expressional profile in the liver.In a combined strategy by using Capillary immuno-electrophoresis (CIE) and standard techniques, we have shown a sexually dimorphic expression of PrPC in mice and human liver tissues. Further, we showed a significant age dependent up-regulation of PrPC expression in the liver of 14 and 9 month-old mice as compared to 3 months of age. Therefore, this study may provide new insights into the gender specific role of PrPC in the liver which may further be linked to its protective role against oxidative stress during aging. In addition, the current study also shows an application of CIE with a low co-efficient of variation to analyze the miniscule amount of PrPC in the mouse liver tissue.This article is protected by copyright. All rights reserved
    Electrophoresis 09/2015; DOI:10.1002/elps.201500244

  • Electrophoresis 08/2015;
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    ABSTRACT: To realise portable systems for routine measurements in point-of-care settings, microchip electrophoresis (ME) methods are required to be robust across many single-use chips. While it's well-known internal standards (ISTDs) improve run-to-run precision, a systematic investigation is necessary to determine the significance of chip-to-chip imprecision in ME and how ISTDs account for this. This paper addresses this question by exploring the reproducibility of Na quantification across six basic, in-house fabricated microchips. A dataset of 900 electrophoerograms was collected from analysing five concentrations of NaCl with two ISTDs (CsCl and LiCl). While both improved the peak area reproducibility, the Na/Cs ratio was superior to the Na/Li ratio (improving the RSD by a factor of 2 to 4, depending on the Na concentration). We attribute this to the significant variation in microchannel surface properties, which was accounted for by cesium but not lithium. Microchip dimension and detector variations were only a few percent, and could be improved through commercial fabrication over in-house made microchips. These results demonstrate that ISTDs not only correct for intra-chip imprecision, but are also a viable means to correct for chip-to-chip imprecision inherent in disposable, point-of-care ME devices. However, as expected, the internal standard must be carefully chosen. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Electrophoresis 03/2015; 36(6). DOI:10.1002/elps.201400399
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    ABSTRACT: The objective of this study was to characterize the differences in electrophoretic behavior between linear and branched PEG-conjugated proteins. Human growth hormone and alpha-lactalbumin modified by linear or branched PEGs with molecular weight of 10 kDa were analyzed by size-exclusion chromatography (SEC), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), SDS-PAGE, and microchip CGE (MCGE). Chromatographic and mass spectrometric differences between the linear and branched PEG-proteins on SEC and MALDI-TOF MS were small, but their electrophoretic behaviors on SDS-PAGE and MCGE were significantly different. In particular, MCGE showed significant differences in the peak width and the migration times of linear and branched PEG-proteins, in which the branched PEG-proteins exhibited a narrower peak and longer migration time than the linear PEG-proteins. This phenomenon may explain the longer circulation half-life for the branched PEG-proteins observed in previously reported in vivo studies. Consequently, this study indicates that MCGE may be a valuable tool for differentiating linear and branched PEG-proteins. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Electrophoresis 03/2015; 36(6). DOI:10.1002/elps.201400539
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    ABSTRACT: For forensic and population genetic purposes, a total of 125 unrelated volunteers' blood samples were collected from Chinese Bai ethnic minority group to analyze sequence variation of two hypervariable segments HVS-I and HVS-II in the mitochondrial DNA control region. Comparing the HVS-I and HVS-II sequences of the 125 Chinese Bais to the Anderson reference sequence, we found 86 polymorphic loci in HVS-I and 40 in HVS-II in mitochondrial DNA sequences of the Chinese Bai ethnic minority group, which defined 93 and 53 different haplotypes, respectively. Haplotype diversity and the mean pairwise differences were 0.992 ± 0.003 and 6.553 in HVS-I, and 0.877 ± 0.027 and 2.407 in HVS-II, respectively. We defined 4 macrohaplogroups R, M, N and D with the proportions ranging from 9.6% to 40.0%. With the analysis of the hypervariable domain from nucleotide 16180 to 16193 in HVS-I, our study revealed new hyplotypes of sequence variations. In addition, the Fst metric, phylogenetic tree and principal component analysis demonstrated a close genetic relationship between the Bai group and Chinese Han populations from South China, Changsha and Guangdong. The results support that the Bai group is a multi-origin ethnic minority which has merged with the Chinese Han population. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Electrophoresis 03/2015; 36(6). DOI:10.1002/elps.201400493
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    ABSTRACT: Thymidine kinase-1 (TK1) is an important cancer biomarker whose serum levels are elevated in early cancer development. We developed a microchip electrophoresis immunoaffinity assay to measure recombinant purified TK1 (pTK1) using an antibody that binds to human TK1. We fabricated poly(methyl methacrylate) microfluidic devices to test the feasibility of detecting antibody (Ab)-pTK1 immune complexes as a step towards TK1 analysis in clinical serum samples. We were able to separate immune complexes from unbound antibodies using 0.5X phosphate buffer saline (pH 7.4) containing 0.01% Tween-20, with 1% w/v methylcellulose that acts as a dynamic surface coating and sieving matrix. Separation of the antibody and Ab-pTK1 complex was observed within a 5 mm effective separation length. This method of detecting pTK1 is easy to perform, requires only a 10 μL sample volume, and takes just 1 minute for separation. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Electrophoresis 03/2015; 36(5). DOI:10.1002/elps.201400436
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    ABSTRACT: For Gaussian peaks, the migration time of the analyte results as the position of the top of the peak and the zone variance is proportional to the peak width. Similar relations have not yet been derived for the Haarhoff-van der Linde (HVL) function, which appears as a fundamental peak shape function in electrophoresis. We derive the relations between the geometrical measures of the HVL-shaped peak, i.e. the position of its maximum, its width and a measure of its asymmetry, and the respective parameters, a1 , a2 and a3 , of the corresponding HVL function. Under the condition of the HVL-shaped peak, the a1 parameter reflects the true migration time of the analyte, which may differ from the peak top position significantly. Our procedure allows us to express the parameters without the need of any external data processing (nonlinear regression). We demonstrate our approach on simulated peaks and on experimental data integrated by the ChemStation software (delivered with the CE instrumentation by Agilent Technologies). A significant improvement is achieved reading the migration time of the experimental and simulated peaks, which draws the error of the HVL-shaped peak migration time evaluation down to the resolution of the data sampling rate. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Electrophoresis 03/2015; 36(5). DOI:10.1002/elps.201400463
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    ABSTRACT: This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li(+) , reported in 2010 by Floris et al. (Lab. Chip 10 (2010)). With respect to this device, significant improvements inaccuracy,precision, detection limit and reliability have been obtained especiallyby the use of multiple injectionsof one sample on a single chip and integrated data-analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patientsby a self-test is reported. For Li(+) in blood better precisionthan the standard clinical determination for Li(+) was achieved. For Na(+) in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca(2+) and Mg(2+) were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Electrophoresis 03/2015; 36(5). DOI:10.1002/elps.201400428

  • Electrophoresis 03/2015; 36(5):641-641. DOI:10.1002/elps.201570054
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    ABSTRACT: In this manuscript, Langevin Dynamics simulations and Tension-Propagation theory are used to investigate the forced translocation of a polymer from a confining tube through a nanopore situated at one of the tube's ends. The diameter of the tube allows for a control over the polymer conformations: decreasing the tube diameter reduces the number of conformations available to the polymer chain both before and during translocation. As the tube diameter is decreased, the translocation time is observed to increase. Interestingly, while the width of the distribution of translocation times is reduced if the chain starts in a tube, it reaches a maximum for weakly confining tubes. A Tension-Propagation approach is developed for the tube-nanopore setup in the strongly-driven limit. Good agreement between the simulations and the theory allows for an exploration of the underlying physical mechanisms, including the calculation of an effective pore friction and the assessing of the impact of monomer crowding on the trans side. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Electrophoresis 03/2015; 36(5). DOI:10.1002/elps.201400418