European Journal of Applied Microbiology and Biotechnology (Eur J Appl Microbiol Biotechnol )

Publisher: Springer Verlag


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    Applied microbiology and biotechnology
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Springer Verlag

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Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Surface tension, surface viscosity and foaming were investigated as functions of the BSA concentration, salt type, and concentrations of salts and antifoam agent (Desmophen 3600). The same surface properties were measured with defined media of Hansenula polymorpha and Escherichia coli. Salts and the antifoam agent considerably influence the surface properties. The cultivation time and the soluble protein content (above 5 mg l−1) have only a slight effect on the surface properties.
    European Journal of Applied Microbiology and Biotechnology 11/1983; 17(6).
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    ABSTRACT: A p-nitrophenyl-α-D-glucopyranoside-hydrolyzing exo-oligo-1,6-glucosidase (dextrin 6-α-glucanohydrolase, EC. of Bacillus cereus ATCC 7064 was 1,120-fold purified to an electrophoretically- and immunologically-homogeneous form by a simple 4-step method containing as the most efficient step the enzyme elution from immunosorbent with a pH 11 medium including 50% (w/v) glycerol. The final enzyme yield was 47%. The specific activity was 218 μmol p-nitrophenyl-α-D-glucopyranoside hydrolyzed/min/mg protein at 35°C and pH 6.8. The amino-terminal amino acid of the enzyme was determined to be methionine. No antigenic common determinant occurred between this enzyme and each of its homologous counterparts from obligate thermophile Bacillus thermoglucosidius KP 1006 and from facultative thermophile Bacillus coagulans ATCC 7050.
    European Journal of Applied Microbiology and Biotechnology 07/1983; 18(4).
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    ABSTRACT: A flocculent strain of Zymomonas sp. isolated from sugar cane juice was used to produce ethanol (EtOH) from sucrose in a 50 ml upflow floc continuous reactor. A maximun productivity of 92 g EtOH/l per hour (estimated on a total volume basis) with 10% sucrose feed and 60% conversion to ethanol was achieved at a dilution rate (D) of 3 h-1. At D=2 h-1 the productivity was 80 g EtOH/l per hour and the yield 0.4 g EtOH/g sucrose supplied. Dilution rates up to 7 h-1 were assayed and even when no wash out occurred, productivity dropped to 63 g EtOH/l per hour and conversion was very low (16%). These results are compared with those obtained using Zymomonas mobilis ATCC 10988 under the same conditions.
    European Journal of Applied Microbiology and Biotechnology 01/1983; 18(3):186-188.
  • European Journal of Applied Microbiology and Biotechnology 01/1983; 18(2):128-130.
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    ABSTRACT: A combination of Monod kinetics and diffusion equation was used for evaluating the effectiveness factor for immobilized cells on solid supports. This has revealed that there is an optimal value of biofilm thickness which results in a maximum for the effectiveness factor. The optimal value of biofilm thickness becomes smaller with increasing values for the Thiele modulus. The effectiveness factor increases as the saturation constant increases for a given value of the Thiele modulus. Furthermore, optimal range of biofilm thickness becomes broader with increasing values for the saturation constant.
    European Journal of Applied Microbiology and Biotechnology 01/1983; 18(4):249-253.
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    ABSTRACT: A bacterium capable of growing on tetralin (1,2,3,4-tetrahydronaphthalene) as the sole source of carbon and energy has been isolated from soil of a coal dump. It has been identified as Pseudomonas stutzeri. The organism converts tetralin to 1-tetralol and 1-tetralone. The generation time is 50 h with tetralin, and 1.2 h with salicylate, respectively, as carbon source. Under conditions where the cells grow on tetralin the highest yield of 1-tetralol and 1-tetralone so far obtained is about 3% of the tetralin-input. The oxidation of an alicyclic ring structure by an arene-degrading bacterium is being discussed.
    European Journal of Applied Microbiology and Biotechnology 01/1983; 18(1):6-10.
  • European Journal of Applied Microbiology and Biotechnology 01/1983; 17:365.
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    ABSTRACT: Among four chlorobenzoates tested, only 3-chlorobenzoate and 4-chlorobenzoate were capable of inducing benzoate oxidizing cell activities in Acinetobactercalcoaceticus strain Bs 5, whereas 2-chlorobenzoate and 2,6-dichlorobenzoate were not. With the monochlorobenzoates, this inducing capability decreased with increasing proximity of the chlorine atom to the carboxyl group, i.e. in the order: 4-chlorobenzoate > 3-chlorobenzoate > 2-chlorobenzoate. It is therefore supposed that the induction of benzoate oxidizing cell activities is inhibited primarily be sterical influences of the chlorine substituents of the various chlorobenzoates.
    European Journal of Applied Microbiology and Biotechnology 01/1982; 15(2):138-140.
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    ABSTRACT: p-Aminoazobenzene was degraded by Bacillus subtilis to aniline and p-phenylenediamine by reductive fission of an azo bond. The aniline was then acetylated to acetanilide while the p-phenylenediamine underwent 2 successive acetylations to yield p-aminoacetanilide and p-phenylenediacetanilide. In addition, another pathway was found in Bacillus subtilis in which p-aminoazobenzene was metabolised to p-acetamidoazobenzene.
    European Journal of Applied Microbiology and Biotechnology 01/1982; 15(2):141-143.
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    ABSTRACT: Summary After pulsing steady state cultures ofCandida intermedia with pure sucrose, or sucrose adequately supplemented with minerals (2–4 g/l), high substrate uptake rates and both an elevated oxygen consumption and CO2-production rate were observed.
    European Journal of Applied Microbiology and Biotechnology 01/1982; 15(1):36-40.
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    ABSTRACT: The effects of heavy metals in diverse substrateamended river sediments were studied. Cd2+, Pb2+ and Zn2+ generally had a marked inhibitory effect on the synthesis of amylase, cellulase and urease, on numbers of substrate-hydrolysing bacteria, in all sediments studied. Inhibition increased with increasing metal concentration, and amylase was particularly sensitive. Pb2+ generally had the least effect. We conclude that enzyme synthesis measurements are useful in determining the effects of heavy metals on the degradation of organic pollutants in river sediments.
    European Journal of Applied Microbiology and Biotechnology 01/1982; 15(4):241-245.
  • European Journal of Applied Microbiology and Biotechnology 01/1982; 16:105-109.
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    ABSTRACT: Some of the extract and intracellular enzyme activities in K2nB strain of Saccharomyces cerevisiae that growing in the condition which induce spontaneous cell rupture, were measured. B-1-3-glucanase, invertase, acid phosphatase and active chitin synthetase zymogen showed a reduced activity in ruptured cell while alkaline phosphatase shows no differences in its activity.
    European Journal of Applied Microbiology and Biotechnology 01/1982; 15(3):153-155.
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    ABSTRACT: There are many methods of determining the activity of cellulolytic enzymes and numerous ways of expressing this activity. Since, however, the results obtained in different research centres are almost completely incomparable, an attempt has been made to introduce a uniform system for expressing a unit of enzyme activity. This system is based on the relationship between the degree of enzyme dilution and the enzyme activity.
    European Journal of Applied Microbiology and Biotechnology 12/1981; 11(4):229-233.
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    ABSTRACT: Summary The biomass yields (y) and COD reduction efficiencies (η) of a whey fermentation by Kluyveromyces fragilis were studied in a 100-1 fermenter at various stirrer speeds and lactose concentrations, and compared to those obtained in 10-1 and 15-1 fermenters at constant values of the oxygen transfer coefficient (kLa) and air velocity. The empirical models previously constructed by using the 15-1 fermenter data could be used to predict the yields on the other scales by calculating for each run the 15-1 fermenter which would provide the same oxygen transfer coefficient measured by the sulphite method on each fermenter under study. To make this model independent of stirrer speeds used in each generic fermenter, the effect of aeration and mixing was incorporated into an overall parameter (kLa) and the values of y and η were correlated only with temperature, lactose level and kL a, since these variables were approximately orthogonal. The validity of this model was finally checked against the yields reported by Wasserman et al. (1961) in a 6-m3 fermenter, thus confirming the capability of the model to provide a reliable basis for further scale-up on the production scale.
    European Journal of Applied Microbiology and Biotechnology 09/1981; 13(1):1-9.
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    ABSTRACT: Summary The cultivation of Neurospora crassa, strain 74A, in Neurospora complete medium containing a azo dye, “Vermelho Reanil P8B” from Imperial Chemical Industries showed that after 24 h of contact, most of the dye was removed from the medium. The greatest extent of dye removal (91.3 to 89.1%) was achieved when the concentration of the dye was between 16.0 and 32.0 μg ml−1 which is the range found in industrial wastes. The biomass production was unaffected in the presence of the dye at all the concentrations analysed.
    European Journal of Applied Microbiology and Biotechnology 09/1981; 13(1):64-66.
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    ABSTRACT: Using the model presented in part I, the measured time and spacial variations of process variables were simulated with satisfactory accuracy. Especially the experimentally found minima of the longitudinal dissolved oxygen concentration profiles in the substrate limiting growth range, which are caused by the transition from oxygen transfer limited to substrate limited growth along the tower, can be simulated with great accuracy.
    European Journal of Applied Microbiology and Biotechnology 06/1981; 13(3):145-150.
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    ABSTRACT: The influence of moisture content and temperature during storage of grain on the formation of Fusarium metabolites was studied. Naturally and artificially contaminated barley grain samples were stored at 15%, 25%, and 30% moisture contents, and at temperatures of + 5°C + 25°C, and + 30°C. Time of storage varied between one week and six months and the occurrence of Fusarium species and metabolites was analysed. The only Fusarium metabolite detected was zearalenone. The extent of Fusarium contamination decreased during storage whilst the concentration of zearalenone increased. To avoid the danger of mycotoxicoses, grains must be dryed immediately after harvest and then stored at a low temperature.
    European Journal of Applied Microbiology and Biotechnology 01/1981; 11(4):241-243.