Plant Cell Tissue and Organ Culture (PLANT CELL TISS ORG )

Publisher: Springer Verlag

Description

Plant Cell Tissue and Organ Culture is an international journal publishing original results of fundamental studies on plant cells tissues and organs in vitro. Topics covered include: growth development and differentiation anatomical histological and ultrastructural aspects genetics and breeding physiology and biochemistry micropropagation protoplast cell and tissue culture secondary metabolism biotechnology.

Impact factor 2.61

  • Hide impact factor history
     
    Impact factor
  • 5-year impact
    2.73
  • Cited half-life
    6.60
  • Immediacy index
    0.60
  • Eigenfactor
    0.00
  • Article influence
    0.32
  • Website
    Plant Cell, Tissue and Organ Culture website
  • Other titles
    Plant cell, tissue and organ culture
  • ISSN
    0167-6857
  • OCLC
    7984574
  • Material type
    Periodical, Internet resource
  • Document type
    Journal / Magazine / Newspaper, Internet Resource

Publisher details

Springer Verlag

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Author's pre-print on pre-print servers such as arXiv.org
    • Author's post-print on author's personal website immediately
    • Author's post-print on any open access repository after 12 months after publication
    • Publisher's version/PDF cannot be used
    • Published source must be acknowledged
    • Must link to publisher version
    • Set phrase to accompany link to published version (see policy)
    • Articles in some journals can be made Open Access on payment of additional charge
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Agroinfiltration is an Agrobacterium-mediated transient assay for the analysis of gene function and genetic modification in leaves, flowers and fruit tissues of various plants, and transient RNAi-induced silencing by agroinfiltration has been developed in leaves and fruits of several plant species. Here we report the establishment of a transient hairpin RNAi-induced silencing system for colour modification assay in floral tissues of Dendrobium Sonia ‘Earsakul’, a tropical hybrid orchid with purple and white bi-coloured flowers. Portions of the D. Sonia ‘Earsakul’ anthocyanin-related genes chalcone synthase (DseCHS-B) and dihydroflavonol 4-reductase (DseDFR) which are required for anthocyanin synthesis, were cloned into the hairpin-based RNAi vectors, pSTARGATE and pWATERGATE, under the control of the maize ubiquitin and Arabidopsis Rubisco small subunit (ARbcS) promoters, respectively. Characterisation of DseCHS-B and DseDFR expression profiles revealed that their expression was developmentally regulated in the flower and associated with flower pigmentation. Transient RNA silencing of DseCHS-B and DseDFR in the floral tissues of D. Sonia ‘Earsakul’ was induced by delivering 436-bp DseCHS-B and 470-bp DseDFR hairpin RNAs (hpRNAs) into the sepals and petals of flower buds at early developmental stages using agroinfiltration. Impaired anthocyanin accumulation and reduction of endogenous mRNAs of the corresponding targets were observed in the infiltrated areas of the sepals and petals. Silencing of the endogenous DseCHS-B and DseDFR mRNAs was highly effective at the early stages of mRNA synthesis and anthocyanin accumulation. This transient silencing system is a prototype for modification of the anthocyanin biosynthetic pathway in D. Sonia Earsakul and other orchids through gene suppression.
    Plant Cell Tissue and Organ Culture 02/2015; 120(2).
  • [Show abstract] [Hide abstract]
    ABSTRACT: The phenomenon of hyperhydricity, a physiological disorder occurring frequently in tissue culture, causes ultrastructural modification and metabolic alteration of shoots. Reactive oxygen species (ROS) accumulation and oxidative stress induction are common features during the development of hyperhydricity, but the relationship between organelle redox homeostasis and hyperhydricity with ultrastructural abnormalities is unclear. To investigate the origin of oxidative stress-induced hyperhydricity, changes in oxygen metabolism in different subcellular compartments of garlic plantlets in vitro were studied. Under exogenous hydrogen peroxide (H2O2) stress, the chloroplastic and mitochondrial ultrastructure was disrupted, which was concomitant with aggravated frequency and severity of hyperhydricity. The addition of H2O2 to the growth medium enhanced superoxide anion generation and H2O2 content in the subcellular compartments. Accumulation of ROS was the highest in apoplasts. Compared with control shoots, in apoplasts exogenous H2O2 stimulated a sharp increase in superoxide dismutase activity within 4 days and a sharp increase in ascorbate peroxidase and glutathione reductase activities and in ascorbic acid and glutathione contents after 8 days of H2O2 treatment. In the other subcellular compartments, dramatic improvement of the antioxidant system occurred after 12 days. Thus, the apoplast was the most sensitive compartment among those investigated. Apoplastic ROS might play a signaling role to participate in the coordination of stress adaptation. The apoplastic oxidative burst in garlic plantlets in vitro is an early response to the development of hyperhydricity.
    Plant Cell Tissue and Organ Culture 02/2015; 120(2).
  • [Show abstract] [Hide abstract]
    ABSTRACT: Phosphoinositide-specific phospholipase C (PI-PLC) plays a central role in the phosphatidylinositol specific signal transduction pathway. Plant PI-PLCs have been demonstrated to be involved in various stimuli and physiological processes. Five Arabidopsis thaliana PI-PLC genes were overexpressed transiently in tobacco leaves, and one of them, AtPLC5, was stably expressed in transgenic Arabidopsis; a dexamethasone-inducible promoter was used. Expression of AtPLC5 in transiently transformed tobacco resulted in leaf senescence at 72 h after induction, while other AtPLC did not cause any visible changes. Stable overexpression of AtPLC5 in transgenic Arabidopsis resulted in clearly induced visible yellowing at 8 days after induction, meanwhile chlorophyll content was decreased, PI-PLC activity and electric conductivity were increased. qPCR results demonstrated that increased transcription of senescence-associated genes (SAG12, SAG13, SAG15, SAG18 and SAG29) were up regulated after AtPLC5 induction. When AtPLC5 fused with green fluorescent protein was transiently expressed in protoplasts prepared from Arabidopsis mesophyll cells, green fluorescence was clearly observed in the plasma membrane (PM). Moreover, western blot analysis indicated that AtPLC5 was found to be enriched in the PM fraction of transgenic Arabidopsis stably expressing AtPLC5. These results suggest that AtPLC5 is predominantly localized in the PM and may be a regulator of leaf senescence.
    Plant Cell Tissue and Organ Culture 02/2015; 120(2).
  • Plant Cell Tissue and Organ Culture 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: An in vitro plant regeneration and genetic transformation protocol was established in jute (Corchorus capsularis L. var JRC321). One-day-old apical, meristematic tissues of germinating seedlings were used as explants. Multiple shoots were regenerated from each explant using Murashige and Skoog basal medium containing 1.78 µM benzylamino purine and 4.92 µM indole-3-butyric acid. Transformation was carried out in three independent sets (each set comprising of three independent experiments each comprising three replications with 35 explants per replication) using the bialaphos resistance gene (bar), synthetically designed for high level plant expression. The positive transformants containing the bar gene were selected in growth medium containing 2.5 mg/l bialaphos. Polymerase chain reaction (PCR), Southern and northern blots, real-time quantitative PCR, western blot and enzymatic assay of five putative transformants from three independent sets provided evidence for full-length gene integration into the genomic DNA of transformed jute, as well as high level expression of the transgene. Analysis of the T1 plants revealed a stable inheritance of the transgene through the progenies. The data presented in this report showed considerable advancement in jute transformation and should improve future genetic engineering strategies to be employed for improvement of this very important fibre crop.
    Plant Cell Tissue and Organ Culture 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we established the first efficient method to regenerate diploid apomict Boechera divaricarpa via shoot organogenesis. Hypocotyl explants were cultured on MS medium supplemented with plant growth regulators. The morphogenic potential of B. divaricarpa hypocotyl tissue was investigated to establish an efficient adventitious shoot regeneration system. We tested hypocotyls from 7-day-old in vitro seedlings. The effect of various concentrations of cytokinin and auxin on in vitro regeneration of these explants was also investigated. We found that callus induction and shoot regeneration were significantly affected by the concentrations and types of plant growth regulators. MS medium supplemented with 17.75 µM benzylaminopurine and 0.53 µM naphthaleneacetic acid gave the highest number of shoots per explant after 4 weeks in culture. We also determined the expression levels of three DNA methyltransferase genes under tissue culture conditions to understand their activities during callus induction and shoot regeneration. BdMET1 was expressed at low levels during both callus induction and shoot regeneration, while expression of BdDRM2 was high during callus induction and low during shoot regeneration. BdCMT3 was highly expressed in both hypocotyl explants and seedlings and may be a good candidate to induce epigenetic variations in diploid apomict B. divaricarpa shoots derived from tissue culture. Our study provides an efficient in vitro regeneration method and determines the expression levels during tissue culture of methyltransferase genes; it can be used as a new tool to understand the molecular biology of apomixis in B. divaricarpa.
    Plant Cell Tissue and Organ Culture 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Clonal propagation of Quercus suber via somatic embryogenesis is an alternative to conventional tree propagation methods; however, complete maturation of somatic embryos is considered the major bottleneck for mass propagation of Quercus species. During somatic embryogenesis, embryo development and maturation are controlled by signaling pathways that integrate information from genetic and epigenetic programs as well as hormonal signals. Therefore, in this study genes were identified related to epigenetic regulation and the abscisic acid (ABA) pathway during development and maturation of cork oak somatic embryos. A total of eight expressed sequence tags (ESTs) were obtained of genes encoding a 9-cis-epoxycarotenoid dioxygenase (NCED), two histone deacetylases (HDA6 and HDA19), two histone monoubiquitinases (HUB1 and HUB2), a histone H3 kinase (AUR3) as well as genes related to chromatin remodeling processes PICKLE and VP1/ABSCISIC ACID INSENSITIVE 3-LIKE 1 (VAL1). The analysis of the expression patterns of selected genes during different developmental stages indicated that QsNCED3 may play a role in ABA synthesis during embryogenesis. The change in the expression levels for all seven genes associated with epigenetic regulation showed that QsHUB1 and QsHUB2 may have a role in ABA signalling while QsHDA6 and QsHDA19 could act in different pathways than in Arabidopsis. Furthermore, expression levels of QsAUR3 indicated that histone phosphorylation is an early epigenetic mark in Q. suber somatic embryos while QsPICKLE and QsVAL1 may be necessary for the correct development of cork oak somatic embryos.
    Plant Cell Tissue and Organ Culture 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Passiflora is a large and widespread genus of tropical plants that includes over 500 species. Organogenesis-based in vitro plant regeneration systems have long been available for the commercially important species Passiflora edulis, the passionfruit, and for a few other related wild species. Recently, somatic embryogenesis from mature zygotic embryos was reported for passionfruit and for a related wild species, P. cincinnata, although the recovery of entire plants was obtained only for the latter. Here we assessed the in vitro morphogenic responses of zygotic embryos of five different Passiflora species (P. alata Curtis, P. crenata Feuillet & Cremers, P. edulis Sims, P. foetida L. and P. gibertii N.E. Brown) cultured in basal Murashige and Skoog (MS) medium supplemented with 4.5 μM 6-benzyladenine (BA) and different concentrations (13.6, 18.1, 22.6 or 27.1 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D). We characterized these different responses using light and scanning electron microscopy. Somatic embryos were obtained in MS medium supplemented with 4.5 μM BA and either 13.6 or 18.1 μM 2,4-D for all species, except P. foetida for which only indirect shoot organogenesis was observed. Regeneration of entire plants that could be acclimatized was achieved for all species studied. Additionally, our results indicated that the in vitro conditions that promote somatic embryogenesis in some Passiflora species might induce shoot organogenesis in others, suggesting that the conservation of morphogenetic signals among Passiflora species might be limited by their phylogenetic relatedness.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: We have induced callus tissues from one R6/R6 homozygous genotype red-fleshed apple individual which was the hybrid offspring of Malus sieversii f.niedzwetzkyana and ‘Fuji’, and investigated the effect of auxin alone and auxin combined with cytokinin or nitrogen deficiency on anthocyanin synthesis. In callus culture, auxin alone significantly inhibited anthocyanin biosynthesis with the increase of auxin concentration. The inhibitory effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on anthocyanin accumulation was about tenfold stronger than naphthalene acetic acid. Anthocyanin regulatory genes (MdMYB10 and MdbHLH3) and structural genes were dramatically suppressed by 0.6 mg/L 2,4-D. The inhibitory effect of auxin on anthocyanin biosynthesis was influenced by cytokinins 6-benzylaminopurine (BAP) and thidiazuron (TDZ) as well as nitrogen deficiency. Auxin and cytokinin displayed the interaction in controlling anthocyanin biosynthesis. Co-treatment of auxin and cytokinin (BAP or TDZ) significantly enhanced the cytokinin-induced increase in anthocyanin levels but too high auxin concentration strongly inhibited anthocyanin synthesis even in the presence of cytokinin. Nitrogen deficiency could reverse the inhibition of anthocyanin accumulation by auxin.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: Tissue-specific promoters for efficient expression of transgenes at specific times or in specific plant tissues can be applied to develop new transgenic plants. To exploit a promoter capable of driving strong expression in potato tubers, we isolated the promoter region of the laccase gene from potato (Solanum tuberosum L. cv. Desiree) and characterized its activity in transgenic Solanaceae plants, such as potato, tobacco and tomato. The ability of the laccase promoter to induce the β-glucuronidase (GUS) reporter was evaluated in independent transgenic potato lines and compared with that of the constitutive CaMV35S promoter. To determine the tissue specificity of expression in transgenic potato, GUS levels in shoot tips, leaves, stems, roots and tubers were measured by histochemical analysis. The laccase promoter conferred tuber-specific expression in transgenic potato regardless of the developmental stage, and there was no GUS reporter expression in leaves, stems or roots. Serial 5′ deletion analysis of the laccase promoter revealed that the tuber-specific regulatory elements might be scattered throughout the promoter. The laccase promoter responded weakly to salt stress, mannitol stress, and mechanical wounding but not to cold stress in the leaves and stems of transgenic potato. In transgenic tobacco, weak GUS expression driven by the laccase promoter was detected throughout the entire plant, whereas in transgenic tomato, GUS expression was detected only in the roots and seeds. Our data show that the laccase promoter represents a feasible candidate to drive high and preferential expression of genes in potato tubers.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: Salicornia brachiata Roxb. (Amaranthaceae) a leaf-less annual succulent halophyte, grows under extremely saline conditions and is an important resource for salt stress responsive genes. Here we report somatic embryogenesis and in vitro plantlet regeneration in S. brachiata for the first time. In vitro-grown seedlings were used as explants and 86.0 ± 1.7 % explants produced embryogenic callus on 0.75 % agar-gelled Murashige and Skoog’s (MS) medium containing 2.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-d) in 16 weeks. Somatic embryogenesis was achieved on MS medium supplemented with 0.25 mg l−1 2,4-d. On this medium sustained cell division and growth within the first 8 weeks resulted in the formation of cell aggregates in 73.65 ± 0.44 % cultures producing globular somatic embryos (SEs). Gradually these converted into heart, torpedo and cotyledonary shaped SEs following subsequent 16 weeks subculture on the same medium. Around 35 % SEs germinated on plant growth regulator free MS medium. The somatic seedlings were acclimatized on sterile soil with 40–45 % survival.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of work was to elaborate the system for Agrobacterium tumefaciens-mediated transformation and transgenic plant regeneration in Rubus fruticosus L. using explants from mature plants. Regeneration of transgenic shoots was achieved from cut ends of petioles completely immersed in the medium using flag explants cultivated vertically on MS medium with 1 mg l−1 TDZ and 0.02 mg l−1 IBA followed by transfer on medium with 1 mg l−1 BAP, 0.02 mg l−1 IBA and 0.1 mg l−1 GA3, both supplemented with 10 and 15 mg l−1 hygromycin after transformation by A. tumefaciens strain LBA 4404/pCambia 1304. Four putative transgenic plants of cv. ‘Čačanska Bestrna’ were rooted, acclimatized and analysed. Transgenic character of the tissue from analysed plants was confirmed by nested PCR and subsequent sequencing.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: RNA interference (RNAi) is a novel method of gene regulation and one of the potent host defense mechanisms against viruses. It however acts as a deterrent in transgene-technology by constraining the expression of the introduced gene. The virus-encoded suppressors have the ability to restrict host RNAi to promote pathogenicity. They thus have tremendous potential to ameliorate low transgene expression and have important applications in the biofarming sector. Unfortunately the suppressors severally reduce plant regeneration potentials in the standard procedures. In this study, we report a simple, fast and efficient method for in planta transformation of rice seeds that can be used for over-expressing FHVB2, a well-characterized suppressor of RNAi. The protocol involves agro-inoculation of embryos without vacuum infiltration or injury followed by their growth ex vitro. Following transformation the transgene integration, expression and stable inheritance was confirmed. We observed that the FHVB2 transgenic survival in this methodology was 15-fold higher compared to that in available callus-based methods. The protocol has the potential to be extended for transforming rice with any gene as exemplified by the use of control constructs.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: The GH3 family is an important class of early auxin-response genes involved in the development of the hypocotyls and roots in Arabidopsis thaliana, but the role of this gene family in woody plants is poorly understood. In this study, we cloned a GH3-like gene from Betula platyphylla × Betula pendula (birch) named BpGH3.5 and produced transgenic birch lines that overexpressed either a sense or antisense version of the BpGH3.5 gene using Agrobacterium-mediated transformation. We found that both types of transgenic lines exhibited short primary and lateral roots in vitro, which was caused by a smaller sized root apical meristem with a fewer cells compared with the non-transgenic plant as observed in paraffin sections. The qRT-PCR results showed that the expression of genes associated with auxin and cytokinin metabolism and signaling changed in the transgenic lines. These results indicated that cytokinin and auxin crosstalk caused a small meristem, short-root phenotype in BpGH3.5 transgenic lines. In addition, transgenic sense and antisense BpGH3.5 lines showed reduced indole-3-acetic acid and N-1-napthylphthalamic-acid sensitivity. Taken as a whole, our results suggested that BpGH3.5 had a complex function and sophisticated mechanism of regulation in woody plants.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: Widening the genetic base of minor grain legume crops such as lentil (Lens culinaris Medik.) is important for achieving future gains in productivity. In order to access genes from wild lentil species embryo rescue techniques are required to overcome reproductive barriers. Removing the seed coat from developing 14-day old immature hybrid seeds and culturing the interspecific zygotic embryos in medium containing auxin 4-chloroindole-3 acetic acid (4-Cl-IAA) improved successful hybrid recovery. Addition of 4-Cl-IAA to media also increased shoot proliferation when combined with a low concentration of zeatin. No significant difference in shoot elongation was observed between 4-Cl-IAA or IAA treatments. Hybrid shoots were then successfully grafted in vivo onto faba bean rootstocks. Hybrids were obtained from crosses of L. culinaris with L. tomentosus Ladiz., L. lamottei Czef., and L. odemensis Ladiz. This efficient and simple embryo rescue protocol resulted in seed production of large interspecific F2 populations from inherently weak zygotic embryos produced from wide hybridization.
    Plant Cell Tissue and Organ Culture 01/2015; 120(1).
  • [Show abstract] [Hide abstract]
    ABSTRACT: An efficient protocol for plant regeneration was developed from protoplasts of Gentiana macrophylla Pall. through somatic embryogenesis. Viable protoplasts were isolated from cell suspensions derived from young seedling leaves in an enzyme solution containing 2 % Cellulase Onozuka R-10, 0.5 % Macerozyme R-10, 0.5 % Hemicellulase, and 0.4 M sorbitol with a yield of 6.2 × 106 protoplasts g−1 fresh weight. Liquid, solid–liquid double layer (sLD) and agar-pool (aPL) culture systems were used for protoplast culture. The frequency of protoplast cell divisions and colony formations in aPL culture were significant (p aPL culture supplemented with 2 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-d) and 0.5 mg l−1 6-benzylaminopurine (BA). Protoplast-derived microcalli obtained from aPL culture system were transferred to solid MS medium with a reduced concentration of 2,4-d (0.5 mg l−1) to promote the formation of embryogenic calli. Somatic embryos developed into plantlets on MS medium supplemented with 2 mg l−1 BA at a rate of 51.3 %. RAPD analysis of G. macrophylla revealed a low variation among regenerants.
    Plant Cell Tissue and Organ Culture 01/2015;