Journal of Inorganic Biochemistry (J INORG BIOCHEM)

Publications in this journal

• Article: Copper(II) mixed chelate compounds induce apoptosis through reactive oxygen species in neuroblastoma cell line CHP-212

  Anllely Grizett Gutiérrez, Adriana Vázquez-Aguirre, Juan Carlos García-Ramos, Marcos Flores-Alamo, Enrique Hernández-Lemus, Lena Ruiz-Azuara, Carmen Mejía
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  ABSTRACT: In the present work we report the antiproliferative activity of Cu(II) coordination compounds, CasIIgly ([Cu(4,7-dimethyl-1,10-phenanthroline) (glycinato) (H2O)]NO3), CasIIIia ([Cu(4,4'-dimethyl-2,2'-bipyridine) (glycinato) (H2O)]NO3), and CasIIIEa ([Cu(4,7-dimethyl-1,10-phenanthroline) (acetylacetonato) (H2O)]NO3), against human tumoral cell line CHP-212 (estromal neuroblastoma). Additionally, the molecular structure of CasIIIEa was reported. The IC50 values obtained for the evaluated compounds are in the range 18 to 47 μM, representing an inhibition potency increase of 5 to 12 times compared with cisplatin (IC50 = 226.7 μM). After 2 h of incubation with the evaluated compounds, cells showed high levels of reactive oxygen species and a considerable GSH depletion, besides an important disruption of the mitochondrial membrane with release of cytochrome C and besides the presence of caspase-3, an effector caspase that is activated in the last step of apoptosis cascade. The results confirm that cell death in neuroblastoma CHP-212 treated with Casiopeínas occurs via apoptosis. Due the lack of expression of caspase-8, cell death is principally by the mitochondrial pathway. Thus, one of the most interesting findings of this work is the identification of a very important damage in neuroblastoma cells induced by Cu(II) coordination compounds in a very short exposition times.
  Journal of Inorganic Biochemistry 05/2013;
• Article: A QM/MM refinement of an experimental DNA structure with metal-mediated base pairs

  Sadhana Kumbhar, Silke Johannsen, Roland K.O. Sigel, Mark P. Waller, Jens Müller
  Journal of Inorganic Biochemistry 03/2013;
• Article: Biological activity and cellular uptake of [Ru(η5-C5H5)(PPh3)(Me2bpy)][CF3SO3] complex

  Tânia S. Morais, Filipa Santos, Leonor Côrte-Real, Fernanda Marques, M. Paula Robalo, Paulo J. Amorim Madeira, M. Helena Garcia
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  ABSTRACT: Anticancer activity of the new [Ru(η5-C5H5)(PPh3)(Me2bpy)][CF3SO3] (Me2bpy = 4,4′-dimethyl-2,2′-bipyridine) complex was evaluated in vitro against several human cancer cell lines, namely A2780, A2780CisR, HT29, MCF7, MDAMB231 and PC3. Remarkably, the IC50 values, placed in the nanomolar and sub-micromolar range, largely exceeded the activity of cisplatin. Binding to human serum albumin, either HSA (human serum albumin) or HSAfaf (fatty acid-free human serum albumin) does not affect the complex activity. Fluorescence studies revealed that the present ruthenium complex strongly quench the intrinsic fluorescence of albumin. Cell death by the [Ru(η5-C5H5)(PPh3)(Me2bpy)][CF3SO3] complex was reduced in the presence of endocytosis modulators and at low temperature, suggesting an energy-dependent mechanism consistent with endocytosis. On the whole, the biological activity evaluated herein suggests that the complex could be a promising anticancer agent.
  Journal of Inorganic Biochemistry 02/2013; 122(May 2013):8-17.
• Article: "[RuII(5-C5H5)(bipy)(PPh3)]+, a promising large spectrum antitumor agent: cytotoxic activity and interaction with Human Serum Albumin"

  Tomaz
  Journal of Inorganic Biochemistry 07/2012;
• Article: Cytotoxic copper(II), cobalt(II), zinc(II), and nickel(II) coordination compounds of clotrimazole

  Soledad Betanzos-Lara, Celedonio Gómez-Ruíz, Lidia R. Barrón-Sosa, Isabel Gracia-Mora, Marcos Flores-Álamo, Noráh Barba-Behrerns
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  ABSTRACT: Sixteen novel mononuclear Cu(II), Co(II), Zn(II), and Ni(II) complexes of the biologically active ligand clotrimazole (clotri) of the form [M(clotri)2Cl2]•nH2O (1–4), [M(clotri)2Br2]•nH2O (5–7), [M(clotri)3Br2] (8), [M(clotri)3NO3]NO3•nH2O (9, 11), [M(clotri)3(NO3)2]•nH2O (10), and [M(clotri)3(OH2)2NO3]NO3•nH2O (12) were synthesized and fully characterized. Dinuclear [Cu2(clotri)4µ2-Cl4]•2H2O (1a) and [Cu2(clotri)4µ2-Br2]•2H2O (5b) as well as tetranuclear [Cu4(clotri)4µ4-Br6µ4-O] (5a) complexes were also isolated. Complexes 1‒7, 9, and 11 present a tetrahedral geometry; complex 8 exhibits a pentacoordinated structure; complexes 1a, 10 and 12 an octahedral geometry. X-Ray crystal structures of [Cu(clotri)2Cl2]•(1), [Cu(clotri)2(EtOH)Cl2]•(1•EtOH), [Zn(clotri)2Cl2] (3), [Zn(clotri)2Br2] (7), and [Cu4(clotri)4µ4-Br6µ4-O] (5a) were obtained. Complexes 1–12 were tested for cytotoxic activity against the human carcinoma cell lines HeLa (cervix-uterine), PC3 (prostate), and HCT-15 (colon) with IC50 values < 30 µM. Confocal microscopy and nuclear dying (DAPI) for complex 1 showed condensation of cromatin and nuclear membrane fragmentation. Immunocytochemical detection/expression of biomarkers suggests that complexes 1 and 9 induce cell death via apoptosis. TUNEL assay detected DNA fragmentation in HeLa cells, resulting from apoptotic signaling cascades induced by Cu(II) complexes 1 and 9. 1H NMR studies of the Zn(II) complexes showed that they can bind to nucleotides.
  Journal of Inorganic Biochemistry 05/2012;
• Article: DNA Conformation and Repair of Polymeric Natural DNA Damaged by Antitumor Azolato-Bridged Dinuclear PtII Complex

  Mlcouskova, Kasparkova, Suchankova, Komeda, Brabec
  Journal of Inorganic Biochemistry 05/2012;
• Article: Overexpression, purification, and biochemical and spectroscopic characterization of coppercontaining nitrite reductase from Sinorhizobium meliloti 2011. Study of the interacti

  Félix M. Ferroni, Sergio A. Guerrero, Alberto C. Rizzi, Carlos D. Brondino
  Journal of Inorganic Biochemistry 04/2012;
• Article: Synthesis of organometallic ruthenium(II) complexes with strong activity against several human cancer cell lines

  Tânia S. Morais, Tiago J.L. Silva, Fernanda Marques, M. Paula Robalo, Fernando Avecilla, Paulo J. Amorim Madeira, Paulo J.G. Mendes, Isabel Santos, M. Helena Garcia
  Journal of Inorganic Biochemistry 02/2012;
• Article: Ferraroni M., Matera I., Chernykh A., Kolomytseva M., Golovleva L., Scozzafava A., Briganti F. Reaction Intermediates and Redox State Changes in a Blue Laccase from Steccherinum ochraceum observed by Crystallographic High/Low X-Ray Dose Experiments. J Inorg Biochem, 2012, V. 111, pp. 203-209.

  Ferraroni M, Matera I, Chernykh A, Kolomytseva M, Golovleva L, Scozzafava A, Briganti F
  Journal of Inorganic Biochemistry 01/2012;
• Article: Diastereomerically Pure Platinum (II) Complexes as Antitumoral Agents. The influence of the mode of binding {(N),(N, O)< sup>-</sup> or (C, N)}< sup>-</sup> of (< i> 1S, 2R )< i>- [(η< sup> 5</sup>-C< sub> 5</sub> H< sub> 5</sub>) Fe {(η< sup> 5</sup>-C< sub> 5</sub> H< sub> 4</sub>)-CH= N-CH (Me)-CH (OH)-C< sub> 6</sub> H< sub> 5</sub>}] and the arrangement of the auxiliary ligands

  D. Talancón, C. López, M. Font-Bardía, T. Calvet, J. Quirante, C. Calvis, R. Messeguer, R. Cortés, M. Cascante, L. Baldomá
  Journal of Inorganic Biochemistry 01/2012;
• Article: Novel estradiol based metal complexes of Tc-99m

  Carina Neto, Maria Cristina Oliveira, Lurdes Gano, Fernanda Marques, Thies Thiemann, Isabel Santos
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  ABSTRACT: Aiming to contribute to the design of technetium imaging agents for estrogen receptor (ER) positive breast tumors, we have synthesized and evaluated the novel organometallic estradiol complexes (fac-[M(CO)3 (κ3-10)]+ and fac-[M(CO)3(κ3-12) M=Re/99mTc) using two different bifunctional tridentate ligands (4 and 8). The rhenium complexes (13 and 14) were fully characterized by IR, 1H NMR, 13C NMR, mass spectrometry and elemental analyses. The 99mTc complexes (15 and 16) were obtained with high radiochemical purity and exhibited high in vitro radiochemical stability. To get a first insight into the relevance of these complexes for targeting ER positive tumors, ER binding affinity assays and cellular internalization studies in an ER expressing cell line, MCF-7, have also been performed suggesting a non ER mediated uptake
  Journal of Inorganic Biochemistry 01/2012; 111:1-9.
• Article: nataraj chitrapriya

  Nataraj Chitrapriya a, Yoon Jung Jang b, Seog K. Kim b, Hyosun Lee a
  Journal of Inorganic Biochemistry 01/2011; 105:1569.
• Article: Accumulation of Eu3+ chelates in cells expressing or not P-glycoprotein: implications for blood-brain barrier crossing

  Darghal N, Garnier-Suillerot A, Bouchemal N, Gras G, Geraldes CF, Salerno M
  Journal of Inorganic Biochemistry 01/2010; 104(1):47-54.
• Article: Antiproliferative activity and QSAR study of copper(II) mixed chelate [Cu(N–N)(acetylacetonato)]NO3 and [Cu(N–N)(glycinato)]NO3 complexes, (Casiopeínas®)

  María Elena Bravo-Gómez, Juan Carlos García-Ramos, Isabel Gracia-Mora, Lena Ruiz-Azuara
  Journal of Inorganic Biochemistry 01/2009; 103:299.
• Article: Oxindole-Schiff base copper(II) complexes interactions with human serum albumin: spectroscopic, oxidative damage, and computational studies

  V. C. da Silveira, G. F. Caramori, M. P. Abbott, M. B. Gonçalves, H. M. Petrilli, A. M. Da Costa Ferreira
  Journal of Inorganic Biochemistry 01/2009; 103:1331.
• Article: Dinuclear organoiridium(III) mono- and bis-intercalators with rigid bridging ligands: Synthesis, cytotoxicity and DNA binding

  Ali Nazif Mhd, Bangert J.-A, Ott I, Gust R, Stoll R, Sheldrick W.S
  Journal of Inorganic Biochemistry 01/2009; 103(10):1405-1414.