Egyptian dental journal Impact Factor & Information

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ISSN 0070-9484

Publications in this journal

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    ABSTRACT: Abstract: Aim: To determine the best enzymatic method for collagen extraction from the Egyptian Nile Tilapia fish scales. Methodology: Fish scales were collected, cleaned and demineralized using HCl solution. Two different techniques for fish scale collagen extraction were conducted, heating scales at 121 ºC prior to extraction, or extraction at 4 ºC. Extraction at 4 ºC was performed using two different enzymes, pepsin and papain, with two different concentrations for each. Results: Heating of scales at 121 ºC resulted in denaturation of collagen. Pepsin enzyme with 1 wt. % concentration was more effective for collagen extraction compared to papain enzyme of equal or even higher concentration. The results of Sodium dodecyl sulfate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed that extracted collagens were type I collagen composed of at least two different α-chains with the α chain composition of (α1)2 α2 and (β) component as well. Conclusion: Nile Tilapia scales can be used as an effective source for type I collagen extraction, being the first time in Egypt to extract collagen from fish scales, with the most effective method is by using Pepsin of 1 wt. % of demineralized scales at 4 ºC.
    Egyptian dental journal 01/2015; 61:327:332.
  • Egyptian dental journal 01/2015;
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    ABSTRACT: In Saudi Arabia, decayed, missing, and filled teeth (dmft) score in the adult and young populations continues to be high (5.38 for primary teeth and 3.34 for permanent ones). Therefore, the need for proper detection and diagnosis of early carious lesion is crucial. Purpose: In this study, we evaluated senior students, Interns and fresh graduates and postgraduates’ awareness and familiarization with the latest trends in caries detection. Materials and Methods: A questionnaire was formulated to evaluate senior dental students, interns, fresh graduates and postgraduates’ awareness and knowledge regarding methods of caries detection. The Questionnaire was distributed in Jeddah, Saudi Arabia. Results: 154 participants answered the questionnaire. Responses expressed low utilization of the International Caries Detection and Assessment System (ICDAS) (7.4%) or adjunct advanced technologies as methods for caries detection. Lack of knowledge or availability issues were reported. Post-graduate dentists never use newer methods for detection or even ICDAS. This finding is disappointing because they are highly educated participants. Conclusion: It was clear that proper implementation of using newer caries detection methods is a must in undergraduate years together with familiarizing students with ICDAS caries detection scale.
    Egyptian dental journal 10/2014; 60(4):4443-4448.
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    ABSTRACT: Bone morphogenetic protein-2 is a potent inducer of de novo bone generation. Large scalestudies using this technique and material had never been ventured in maxillary clefts. This prospective study aims to compare the grafting outcome of rhBMP-2in unilateral cleft lip and palate (UCLP) patients and the iliac crest graft treated cases, as evident by the eruption pattern of the cleft side permanent maxillary canine. Subject and Methods: A total of 16 patients with unilateral cleft lip and palate, with average age 10.5 years were selected and randomly allocated to two groups. Group A (n=8) received BMP-2 and Group B (n=8) underwent iliac traditional crest bone grafting of the alveolar cleft defect. A third control group was selected from the contralateral canines on the non-cleft side of the same patient Group C. Cone beam computed tomography scans before grafting and 11/2 years post-grafting were obtained. The data obtained from the CBCT scanning were imported into the OnDemand 3D software for secondary reconstruction and performing the measurements. The direction of movement of the canine, both in facio-palatal and mesio-distal direction was determined. The width of the alveolar cleft was measured clinically and radiographically. Results: Canines on the side grafted with BMP (Gp A)erupted in a distal direction, while those on the IC side (Gp B) erupted mesially. On the non-cleft side, Gp (C), canines moved mesially and facially. A negative non-significant correlation was found between the width of the cleft grafted with BMP-2 and direction of canine movement. Conclusions: Sparing children from the procedure of iliac crest harvesting is by itself a compelling reason to favour rhBMP2 use. Canines were able to erupt satisfactory in all patients grafted withBMP-2 without the risk of impaction. However, the indications and optimal timing for Grafting with BMP-2 should be based on factors such as cleft width. Further studies with larger sample size to comprehensively assess the outcome are advised
    Egyptian dental journal 07/2014; 60(july):3053-3060.
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    ABSTRACT: Aim: The study investigated the effect of curing method (with or without light) on flexural strength (FS), flexural modulus (FM) and degree of conversion (DC) of two dual-cured resin composite core-buildup materials. Both dual-cured materials differ in their filler size, amount and distribution. Furthermore, the correlation between DC and flexural properties under different curing methods was also evaluated. Materials and Methods: A total of 80 bar-shaped specimens (25X2X2mm3) were tested in a 3-point bending test (cross-head speed: 1mm/min), after storage under 4 different storage conditions (10 min dry incubator storage (baseline), storage in distilled water for 30 min, 1hr and 24hrs following a 10 min dry storage. Immediately after fracture, specimens were analyzed by FTIR spectrometry (n=5/material and storage condition) evaluating each specimen at 3 spots, with 16 scans/spot) to calculate its degree of conversion (DC). Results: Highly significant differences in flexural strength (FS) and flexural modulus (FM) as well as degree of conversion (DC) were recorded between both curing methods for both core-buildup materials stored under different conditions. Rebilda DC revealed the highest flexural properties for both curing methods (FS:120.10 MPa to 144.00 MPa, FM: 6.83 GPa to 8.81 GPa and FS: 82.40 MPa to 133.30 MPa, FM: 3.30 GPa to 7.73 GPa for the dual-cured and chemically-cured specimens respectively). Luxa Core Z Dual recorded lowest flexural properties, for dual-cured and chemically-cured specimens (FS: 86.30 MPa to 104.50 MPa and 41.20 MPa to 93.90 MPa respectively, FM: 6.40 GPa to 7.91 GPa and 1.64 GPa to 5.31 GPa respectively). However, that material demonstrated highest values of %DC, ranging from 62.34% to 67.16% and from 42.25% to 55.42% for the dual-cured and chemically-cured specimens respectively. A highly significant correlation (p<0.001) between the degree of conversion and both flexural properties was detected only for Rebilda DC. Conclusions: Light-curing as well as filler size, amount and distribution significantly affected both flexural properties as well as %DC of the investigated materials.
    Egyptian dental journal 07/2014; Vol 60(Issue 3):2831-2842.
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    ABSTRACT: Mucogingival surgical operations are designed to provide a functionally adequate zone of keratinized attached gingiva. Although these surgical operations are not specifically designed for pocket elimination, these procedure s can be combined with other operations in an attempt to obtain overall healthy periodontal situation. By definition, mucogingival surgery consists of plastic surgical techniques directed toward the correction of gingivo-mucosal membrane relationships that complicate periodontal disease and may interfere with the success of overall periodontal therapy.
    Egyptian dental journal 04/2014; 60(2):2307-2315.
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    ABSTRACT: Aim: The purpose of the present study was to analyze surface characteristics of Twisted File (TF) Ni-Ti rotary instrument before use and to investigate the effect of instrumentation, irrigation using sodium hypochlorite (NaOCl) and sterilization on surface topography and the elemental composition. Materials and methods: A total of 20 new TF Ni-Ti rotary instruments with size 25 and 6% taper were divided into five groups, five instruments/group Group I, as received files. Group II, files used in preparation of twenty curved mesio-buccal canals (20˚-40˚) in presence of NaOCl. Group III, the five used files in group II were further subjected to a single cycle of steam autoclave sterilization at 121 °C, 15 psi for 30 minutes. Group IV, as received files in contact with 5.25% NaOCl for 15 minutes. Group V, as received files subjected to sterilization, similar to group III. Each instrument was screened from the tip along the working part using the SEM equipped with energy dispersive x-ray microanalysis EDX Unit and representative photomicrographs were taken at a magnification of 500X (surface) and 1000X (blade). The following criteria were recorded: I: Defects: A-On surface(seven): (1) Irregular metal strips (2) Regular metal strips (3) Pitting (4) Crater (5) Machine striations (6) Ballooning along the striations(7) Scrapes. B- On blade edge (Four): (1) Roll-over (2) Pits (3) Notches (4) Localized metal flake. II: Debris. Chi-square (x2) compared percentage of each criterion along the five groups. One-way ANOVA compared mean of sum of all criteria/group or those on surface/group or blade edge/group among the five groups. Significance level was at P ≤ 0.05. Results: There was a statistically significant difference between five groups regarding total number of defects/group (P-value < 0.001), surface defects/group (P-value = 0.001) and blade edge defects/group (P-value = 0.003). Used files in groups II, III had highest mean of total, surface and blade defects (II>III with no significant difference). Eight defects existed at 100%. Unused files in groups IV: NaOCl, V: Sterilization had intermediate mean of total and blade defects and lowest mean of surface defects, (V>IV with no significant difference). Six defects existed in range of 80-100%. Group I had lowest mean of total and blade defects and intermediate mean of surface defects. Three defects existed in 100%. Debris showed 100% prevalence in group I and group IV. EDX of Group I showed: Ni (42%), Ti (33%), Al (11%),C (12%), O (7%), Group II, Ni (49%), Ti (41%), Group III: Ni(50.5%), Ti (41%),Al (8.5%). Group IV: Ni (50%), Ti (42%), Al (8.1%), and Group V: Ni (49%), Ti (41%), Al (8%). Conclusions: 1-Combined mechanical instrumentation and chemical usage of NaOCl with and without subsequent sterilization affected surface topography and elemental composition of rotary Nickel Titanium Twisted Files. 2- Precise manufacturing quality control of as-received Twisted Files instruments is still recommended.
    Egyptian dental journal 01/2014; 60(January):187-199.