Journal of Medical Microbiology Impact Factor & Information

Publisher: Pathological Society of Great Britain and Ireland, Society for General Microbiology

Journal description

The Journal of Medical Microbiology aims to maintain and enhance its high-quality comprehensive coverage of pathogenic micro-organisms and their diseases from research, clinical, and diagnostic points of view. Each issue contains up-to-the-minute editorials, in-depth review articles, original papers presenting research findings, and brief reports and technical notes.

Current impact factor: 2.27

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 2.266
2012 Impact Factor 2.297
2011 Impact Factor 2.502
2010 Impact Factor 2.38
2009 Impact Factor 2.272
2008 Impact Factor 2.19
2007 Impact Factor 2.091
2006 Impact Factor 2.18
2005 Impact Factor 2.318
2004 Impact Factor 2.484
2003 Impact Factor 1.987
2002 Impact Factor 1.779
2001 Impact Factor 1.762
2000 Impact Factor 1.625
1999 Impact Factor 1.735
1998 Impact Factor 1.961
1997 Impact Factor 1.525
1996 Impact Factor 1.935
1995 Impact Factor 1.793
1994 Impact Factor 1.627
1993 Impact Factor 1.522
1992 Impact Factor 1.561

Impact factor over time

Impact factor

Additional details

5-year impact 2.64
Cited half-life 6.60
Immediacy index 0.52
Eigenfactor 0.02
Article influence 0.82
Website Journal of Medical Microbiology website
Other titles Journal of medical microbiology (Online)
ISSN 0022-2615
OCLC 37788051
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Society for General Microbiology

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author cannot archive a post-print version
  • Restrictions
    • 12 months embargo
  • Conditions
    • On institutional repository or open access repository
    • Published source must be acknowledged
    • Version deposited must replicate that accepted for publication (authors version)
    • Set phrase to accompany archived copy (see policy)
    • Publisher's version/PDF cannot be used
    • Publisher last contacted on 29/08/2014
  • Classification
    ​ yellow

Publications in this journal

  • Journal of Medical Microbiology 03/2015; DOI:10.1099/jmm.0.000053
  • [Show abstract] [Hide abstract]
    ABSTRACT: Helicobacter pylori infection is endemic in Korea, and serology testing is widely performed. The aim of this study was to validate and compare the diagnostic accuracy of Korean and Western serologic assays for H. pylori detection in Korean adults. The 114 Korean adults who visited our center over a 6-month period for the evaluation of H. pylori infection using the urea breath test (UBT) were enrolled in this prospective study. Anti-H. pylori IgG was measured using three commercially available immunoassays: Genedia H. pylori ELISA (Green Cross Medical Science Co., Eumseong, Korea), Chorus helicobacter IgG (DIESSE Diagnostica Senese, Siena, Italy), and Vidas H. pylori IgG (BioMérieux, Marcy-l'Etoile, France). Positive UBT findings were obtained in 40.6% of included subjects. The sensitivities and the specificities of Vidas, Chorus and Genedia were 89.7%, 100% and 100% and 85.5%, 75.4% and 80.7%, respectively. We found no differences in sensitivity between the Vidas and Chorus (p=0.125), Chorus and Genedia (p=0.125), and Vidas and Genedia (p=1.000) assays. There were also no differences in specificity between the Vidas and Chorus (p=0.070), Chorus and Genedia (p=0.508), and Vidas and Genedia (p=0.549) assays.In Korean adults, the Genedia H. pylori ELISA, Chorus helicobacter IgG, and Vidas H. pylori IgG assays exhibited a high concurrence rate with similar diagnostic accuracy. Thus, both the Korean and Western noninvasive assays are reliable for serodiagnosis of H. pylori in Korean individuals.
    Journal of Medical Microbiology 03/2015; DOI:10.1099/jmm.0.000050
  • [Show abstract] [Hide abstract]
    ABSTRACT: We report the isolation of a novel helicobacter isolated from the cecum of the Siberian hamster, Phodopus sungorus. Sequence analysis showed 97% sequence similarity to H. ganmani. In addition we report the coinfection of the Siberian hamsters with a Campylobacter sp. and a second helicobacter with 99% sequence similarity to H. sp. flexispira taxon 8 (H. bilis), a species previously isolated from patients with bacteremia. Gross necropsy and histopathology did not reveal any overt pathological lesions of the liver and gastrointestinal tract that could be attributed to the Helicobacter or Campylobacter infections. This is the first helicobacter to be identified in the Siberian hamster and the first report of coinfection of Helicobacter spp. and Campylobacter sp. in asymptomatic Siberian hamsters.
    Journal of Medical Microbiology 03/2015; DOI:10.1099/jmm.0.000051
  • [Show abstract] [Hide abstract]
    ABSTRACT: Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates are the most common group C streptococci in humans, and reports of invasive infections associated with SDSE have been increasing. Molecular epidemiology studies are an important strategy to trace the emergence and spread of possible well-fit bacterial pathogens of humans and animals. In this work, we analysed the antimicrobial and clonal profiles of 115 SDSE infection and colonization isolates of human and equine origin. Pulsed-field gel electrophoresis (PFGE) revealed the spread of two main clusters, clones A (57.4%) and B (26.1%). Remarkably, two isolates from clone B obtained from human colonization cases displayed identical PFGE patterns to those of three equine infection isolates. In addition, multilocus sequence typing allocated these isolates to ST129 (CC31). All of the SDSE isolates were susceptible to penicillin, vancomycin, gentamicin, levofloxacin, and chloramphenicol. Tetracycline and erythromycin resistance rates were 65.2% and 13.9% respectively. Nevertheless, none of the isolates displaying sporadic PFGE patterns showed erythromycin resistance. The majority of erythromycin-resistant isolates from clone A had inducible resistance to macrolides, lincosamines, and streptogramins B (iMLSB phenotype), which is associated with the presence of ermA, whereas the resistant isolates from clone B showed the M phenotype, associated with the mefA gene. In conclusion, the data indicate that the analysed collection of SDSE isolates displayed a clonal structure and that the isolates found in human colonization cases can also be involved in horse infections.
    Journal of Medical Microbiology 03/2015; DOI:10.1099/jmm.0.000052
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Helicobacter pylori (H. pylori) may enter into host cells, maybe as a facultative intracellular pathogen. This study aims to reveal the roles of internalized H. pylori in the bacterial pathopoiesis. Transmission electron microscopic was used to observe the invasion of H. pylori. Invasion rates of H. pylori ( 2 standard strains and 43 clinical strains) were examined by gentamicin invasion assay. The cagA, cagE and vacA genes of H. pylori were detected by PCR. And the cagA 3´region (cagA-EPIYA) of each strains was sequenced. The secretion of IL-8 from AGS and activity of NF-κB induced by intracellular H. pylori were tested by ELISA and the Dual-Luciferase reporter assay system, respectively. It was found that H. pylori could adhere and invade into AGS cells, then continue to survive and multiply in cytoplasm. The average invasion rate of H. pylori gastric cancer plants and that of ulcer plants were both higher than that of gastritis plants (P≈0.0001). And in the clinical strains, cagA, vacA and cagE were all positive, cagA-EPIYA genotypes included ABD 90.7% (39/43) and ABBD 9.3% (4/43), all without comparability. But notablely, average invasion rate of H. pylori vacA s1c-i1-m1b plants was higher than that of vacA s1c-i1-m2 plants (P=0.0445). In addition, the intracellular H. pylori all can induce IL-8 secretion, which would decreased after cells were pretreated with anti-β1-integrin antibody or SN-50 (a NF-κB inhibitor). And the intracellular H. pylori all activated NF-κB, which would be inhibited after cells were pretreated with anti-β1-integrin antibody.
    Journal of Medical Microbiology 03/2015; DOI:10.1099/jmm.0.000049
  • [Show abstract] [Hide abstract]
    ABSTRACT: Norovirus is the leading cause of viral gastroenteritis globally. Norovirus genotype GII.4 is responsible for the majority of outbreaks, but new variants are continuously emerging. The objective of the study was to delineate the clinical manifestations and complications associated with these new norovirus GII.4 variants in children. We investigated norovirus infections from the community outbreak in October 2011-September 2012 and an earlier outbreak in 2006-2007 in northern Taiwan. Norovirus genotypes and their variants were validated using molecular methods. A norovirus outbreak started in mid-2011 and continued through 2012 in northern Taiwan. Hospitalized children infected by norovirus in 2012 showed a significantly higher incidence of intestinal hemorrhage, as indicated by grossly bloody feces (P=0.012) and occult blood in feces (P<0.001), and also presented with more high fever >39℃ (P<0.001), fever >38.5℃ (P<0.001), and fever of any temperature >38℃ (P<0.001), compared to children hospitalized in 2006-2007. Analysis of 20 near-full-length genome sequences indicated an emergence of GII.4 2012 variants in 2011-2012. Circulating noroviruses can be divided into two clusters: GII.4 2012a, which is identical to the newly reported strain GII.4 Sydney 2012; and GII.4 2012b, which is close to GII.4 2006b, the earlier predominant strain. The emerging new variants of norovirus GII.4 caused a distinct clinical syndrome of acute gastroenteritis with severe fever and a high rate of intestinal hemorrhage in children. The genetic diversity associated with changing clinical manifestations poses major obstacles to norovirus control.
    Journal of Medical Microbiology 03/2015; DOI:10.1099/jmm.0.000046
  • [Show abstract] [Hide abstract]
    ABSTRACT: Minocycline (MIN) and tigecycline (TIG) are antibiotics currently used for treatment of multi-drug resistant nosocomial pathogens. In this work, we show that blue light, as well as white light, modulate susceptibility to these antibiotics in a temperature-dependent manner. The modulation of susceptibility by light depends on the content of iron, resulting an increase in iron in a reduction in antibiotic susceptibility both under light as well as in the dark, though the effect was more pronounced in the latter condition. We further provide insights into the mechanism by showing that reduction in susceptibility to MIN and TIG induced by light is likely triggered by the generation of 1O2, which, by an yet unknown mechanism, would ultimately lead to the activation of resistance genes such as those coding for the efflux pump AdeABC. The clinical relevance of these results may rely in surface-exposed wound infections, given the exposure to light in addition to the relatively lower temperatures recorded in these type or lesions. We further show that the modulation of antibiotic susceptibility not only occurs in A. baumannii but also in other microorganisms of clinical relevance such as Escherichia coli or Staphylococcus aureus. Overall, our findings allow us to suggest that MIN and TIG antibiotic treatments may be improved by the inclusion of an iron chelator, a condition that in addition to keeping the wounds in the dark would increase the effectiveness in the control of infections involving these microorganisms.
    Journal of Medical Microbiology 03/2015; DOI:10.1099/jmm.0.000048
  • [Show abstract] [Hide abstract]
    ABSTRACT: Chlamydia psittaci (C. psittaci) is prevalent in chicken broiler production. However, the role of C. psittaci in the respiratory disease complex needs to be clarified. It was our purpose to identify the time point when a C. psittaci infection appeared on a broiler farm and to examine the presence of other respiratory pathogens at that time. We focused on the 'major' respiratory pathogens occurring in Belgian broilers namely Infectious bronchitis virus (IBV), Avian metapneumovirus (aMPV), Ornithobacterium rhinotracheale (O. rhinotracheale), Mycoplasma gallisepticum (M. gallisepticum) and Mycoplasma synoviae (M. synoviae) and examine their co-occurrence with C. psittaci on 3 commercial broiler farms. For all farms, one-day old broilers showed high maternal antibody titers against C. psittaci in the presence of viable C. psittaci. Maternal antibodies seemed to protect against respiratory signs. Maternal antibodies declined and clinical outbreaks could be serologically noticed even before maternal antibodies completely disappeared. Mixed infections with genotypes B/C and B/C/D were observed. Broilers with C. psittaci antibody increases showed conjunctivitis, signs of upper respiratory disease and dyspnoea. C. psittaci always preceded an O. rhinotracheale infection. Infections with aMPV, IBV or Mycoplasma spp were not observed. Evidence was provided that C. psittaci can occur at early age in broilers without a predisposing respiratory infection. Both C. psittaci and O. rhinotracheale should be considered when developing prevention strategies for respiratory disease in broilers.
    Journal of Medical Microbiology 02/2015; DOI:10.1099/jmm.0.000047
  • [Show abstract] [Hide abstract]
    ABSTRACT: Considering the prevalence of L. santarosai infections in Americas and the scarce information about that species, we aimed to apply a multi locus variable number tandem repeat (VNTR) analysis (MLVA) for the molecular typing of L. santarosai isolates from various sources. Amplification of three VNTR loci selected from the L. santarosai genome sequences resulted in a wide range of sizes of the amplified products among the 21 L. santarosai strains. This suggests a variation in tandem-repeat copy numbers in the VNTR loci. The secY sequencing also showed a high nucleotide diversity confirming the MLVA data. In conclusion, this novel MLVA provides a high level of discrimination between L. santarosai isolates and this new typing tool can be used to investigate leptospirosis in regions where L. santarosai predominates.
    Journal of Medical Microbiology 02/2015; DOI:10.1099/jmm.0.000045
  • [Show abstract] [Hide abstract]
    ABSTRACT: Episodes of diarrhoea lead to approximately 14% of outpatient visits and 16% of hospital admissions, and account for on average 35 days of illness per year in children under five years of age. This study examined the causative agents of diarrhoea in children under five years of age in suburban areas of Khartoum, Sudan. A total of 437 stool samples obtained from children with diarrhoea were examined by culture and polymerase chain reaction (PCR) for bacteria, by microscopy and PCR for parasites and by immunoassay for detection of Rotavirus A. Of the 437 samples analysed, 211 (48%) tested positive for diarrheagenic Escherichia coli, 96 (22%) for Rotavirus A, 36 (8%) for Shigella spp., 17 (4%) for Samonella spp., 8 (2%) for Campylobacter spp., 47 (11%) for Giardia intestinalis and 22 (5%) for Entamoeba histolytica. Chloramphenicol showed efficacy in 211 (100%) of isolates of E. coli and Salmonella, and 30 (83%) of isolates of Shigella. Gentamycin showed efficacy in 17 (100%) of isolates of Salmonella, 200 (94%) of isolates E. coli and (78%) 28 of isolates Shigella spp. Ampicillin was not an effective antibiotic, since all bacteria showed high resistance. In conclusion, bacteria proved to be the main cause of diarrhea in young children in this study, followed by Rotavirus A and protozoa. Determination of diarrhea aetiology and antibiotic susceptibility patterns of diarrhoeal pathogens and improved hygiene are important for clinical management and controlled strategic planning to reduce the burden of infection.
    Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000043
  • [Show abstract] [Hide abstract]
    ABSTRACT: Biofilms (BF) are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. Microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. We aimed to screen and characterize biofilm formation by different isolates of Candida on removed IUDs, to perform experimental biofilm formation by isolated strains and to examine it by the crystal violet and the XTT reduction assay and scanning electron microscopy (SEM). 56 IUDs were examined for biofilm formation using Sabouraud's dextrose chloramphenicol agar. Identification of suspected colonies by different methods. Antifungal susceptibility to fluconazole and amphotericin B to isolated strains and invitro experimental biofilm formation was done. The biofilm was quantified by crystal violet, XTT reduction assay and SEM. Among the 56 IUDs investigated, 26 were Candida positive (46.4%). Candida albicans was recovered from 15 isolates. Biofilm MIC to fluconazole increased 64 to 1000 times compare to planktonic cells. XTT method was dependant on candida species; Biofilm formation was highest in Candida krusei and Candida glabrata strains followed by Candida albicans and Candida tropicalis respectively. SEM of Candida biofilm revealed a heterogeneous thick biofilm with a mixture of micro-organisms. The main conclusion from this study was, candida non albicans represents more than one half of Candida biofilm. Better understanding of Candida biofilms may lead to the development of novel therapeutic approaches for the treatment of fungal infections especially resistant ones among IUDs users.
    Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000042
  • [Show abstract] [Hide abstract]
    ABSTRACT: The antimicrobial activity of Cetylpyridinium chloride (CPC) and Miramistin (MST) solutions in different concentrations (5 x 10-5 to 0.4%) and a fleece, containing 0.15% CPC, were tested against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli after 30 minutes (solutions) and 60 minutes (fleece) of incubation, respectively. Furthermore, cytotoxic effects of CPC and MST were examined in human keratinocyte (HaCaT) and murine fibroblast (L929) cell lines. A dose of 3 x 10-3% CPC or MST was sufficient to entirely eradicate S. aureus after 30 minutes of incubation. To achieve the same effect higher concentrations against E. coli (0.025% CPC; 0.0125% MST) and P. aeruginosa (0.5% CPC; 0.05% MST) were required. The CPC-fleece showed a high antiseptic effect against all three bacterial strains, though it did not completely eliminate P. aeruginosa. Both substances showed a high cytotoxic impact in higher tested concentrations (CPC: > 3 x 10-3% MST > 8 x 10-4%). CPC showed high antimicrobial potency in low concentrations against S. aureus, accompanied by low cytotoxic (side) effects in these concentrations, while the required minimal concentration to eradicate E. coli and P. aeruginosa emerged as cytotoxic for keratinocytes and fibroblasts. The necessary antibacterial amounts of MST were lower, but also cytotoxic in direct contact to typical human wound cells. Regarding demographic changes and rising bacterial resistance, new effective antiseptics, such as CPC and MST, incorporated in wound dressings without releasing an active substance can help to improve the treatment and healing rates of chronic wounds.
    Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000034
  • [Show abstract] [Hide abstract]
    ABSTRACT: The concept of infertility as a result of asymptomatic microbial colonization of female reproductive tract has been neglected till date. However, increasing incidence of infertility and advanced research has drawn attention towards this idea. Many of these microorganisms have been reported to bring adverse changes in sperm parameters in vitro, but their in vivo potential to cause infertility is still a controversy. The present study was carried out to observe what effect the intravaginal inoculation of spermagglutintaing Serratia marcescens and sperm immobilizing Candida albicans may have in reproductive tract and consequently in fertility outcome. When these strains were intravaginally inoculated into female Balb/c mice at concentrations of 104, 106,108 cfu for 10 consecutive days and mating of mice on day 12, the result showed 100% decrease in fertility in all the groups as compared to control mice receiving PBS. Further, no clinical or histopathological changes were observed in reproductive organs viz. ovary, uterus and vagina suggesting that colonization of genital tract with sperm impairing microorganisms could be a feasible reason for female infertility.
    Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000036
  • [Show abstract] [Hide abstract]
    ABSTRACT: Electrosyneresis and double diffusion are immunoprecipitation techniques commonly used in the serological diagnosis of Farmer's lung disease (FLD). These techniques are reliable but lack standardization. The aim of this study was to evaluate western blotting for the serodiagnosis of FLD. We carried out western blotting with an antigenic extract of Lichtheimia corymbifera, an important etiologic agent of the disease. The membranes were probed with sera from 21 patients with FLD and 21 healthy exposed controls to examine the IgG antibody responses against purified somatic antigens. Given the low prevalence of the disease, 21 patients can be considered as a relevant series. Four bands were significantly more frequently represented in membranes probed with FLD sera (bands at 27.7, 40.5, 44.0 and 50.5 kDa) than those probed with control sera. We assessed the diagnostic value of different criteria alone or in combination. The diagnostic accuracy of the test was highest with the inclusion of at least two of the following criteria: at least five bands on the strip, and the presence of one band at 40.5 or 44.0 kDa. Sensitivity, specificity, positive and negative predictive values were all 81% and the odds ratio was 18.06. Inclusion of bands of high intensity diminished rather than improved the diagnostic value of the test. We conclude that western blotting is a valuable technique for the serodiagnosis of FLD. The industrial production of ready-to-use membranes would enable the routine use of this technique in laboratories and provide reliable and standardized diagnostic results within a few hours.
    Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000037
  • [Show abstract] [Hide abstract]
    ABSTRACT: Bacteriological examinations were conducted of seven Arcanobacterium haemolyticum strains isolated from elderly patients with skin and soft tissue infections, such as cellulitis and skin ulcers. Streptococcus dysgalactiae or Gram-positive cocci were isolated together with A. haemolyticum from all patients. The strains were identified as A. haemolyticum based their being on catalase negative, reverse CAMP positive, and phospholipase D gene positive in the respective tests. Moreover, API Coryne and MALDI-TOF MS confirmed the identification of A. haemolyticum. All strains showed good susceptibility to minocycline, vancomycin, and β-lactam antibiotics, but several strains were resistant to gentamicin and levofloxacin.
    Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000038
  • [Show abstract] [Hide abstract]
    ABSTRACT: Carbapenem resistant Acinetobacter baumannii are a major health problem especially in intensive care units (ICU) worldwide. This study aims to detect the prevalence of Acinetobacter baumannii colonization of the gastrointestinal tract of patients admitted to the intensive care units in two hospitals in Saudi Arabia. In addition, it aims to characterize the molecular mechanisms of carbapenem resistance in such isolates. From January to June 2014, 565 rectal swab specimens were screened for Acinetobacer strains and carbapenem resistance using chromagar Acinetobacter and chromagar KPC agar plates, respectively. Organism identification and susceptibility were detected using vitek2 system. A total of 47 Acinetobacter spp were detected and 35 of them were resistant to carbapenem making the prevalence of Acinetobacter spp 8.3% (47/565) and carbapenem resistance (6.2%, 35/565). The 47 strains had remarkable clonal diversity as revealed by pulse field gel electrophoresis. Using PCR, OXA-51, a chromosomal marker for Acinetobacter baumannii, was detected in 46 strains. OXA-23 beta lactamase was detected in all 35 carbapenem resistant Acinetobacter baumannii. No IMP-, VIM-, SPM-, SIM-, GIM, KPC-, and NDM-beta lactamases were detected in these isolates. OXA-23 is the main mechanism of carbapenem resistance in these isolates. To our knowledge, this is the first study to detect the prevalence of Acinetobacter colonization in the digestive tract of ICU patients in Saudi Arabia. This study reveals the importance of having well established protocols for early identification of these multi drug resistant organisms, optimizing infection control strategies, and having active surveillance studies to reduce morbidity, mortality, and cost.
    Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000033
  • Journal of Medical Microbiology 02/2015; 64(Pt_4). DOI:10.1099/jmm.0.000035