Journal of Medical Microbiology Impact Factor & Information

Publisher: Pathological Society of Great Britain and Ireland, Society for General Microbiology

Journal description

The Journal of Medical Microbiology aims to maintain and enhance its high-quality comprehensive coverage of pathogenic micro-organisms and their diseases from research, clinical, and diagnostic points of view. Each issue contains up-to-the-minute editorials, in-depth review articles, original papers presenting research findings, and brief reports and technical notes.

Current impact factor: 2.25

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 2.248
2013 Impact Factor 2.266
2012 Impact Factor 2.297
2011 Impact Factor 2.502
2010 Impact Factor 2.38
2009 Impact Factor 2.272
2008 Impact Factor 2.19
2007 Impact Factor 2.091
2006 Impact Factor 2.18
2005 Impact Factor 2.318
2004 Impact Factor 2.484
2003 Impact Factor 1.987
2002 Impact Factor 1.779
2001 Impact Factor 1.762
2000 Impact Factor 1.625
1999 Impact Factor 1.735
1998 Impact Factor 1.961
1997 Impact Factor 1.525
1996 Impact Factor 1.935
1995 Impact Factor 1.793
1994 Impact Factor 1.627
1993 Impact Factor 1.522
1992 Impact Factor 1.561

Impact factor over time

Impact factor

Additional details

5-year impact 2.51
Cited half-life 7.40
Immediacy index 0.46
Eigenfactor 0.02
Article influence 0.76
Website Journal of Medical Microbiology website
Other titles Journal of medical microbiology (Online)
ISSN 0022-2615
OCLC 37788051
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Society for General Microbiology

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author cannot archive a post-print version
  • Restrictions
    • 12 months embargo
  • Conditions
    • Author's pre-print on author's personal website, institutional repository or pre-print archive such as BioRxiv
    • Author's post-print on institutional repository or subject repository
    • Published source must be acknowledged
    • Version deposited must replicate that accepted for publication (authors version)
    • Set phrase to accompany archived copy (see policy)
    • Publisher's version/PDF cannot be used
    • Must link to publisher version with DOI
    • Non-commercial use
    • Publisher last contacted on 06/08/2015
  • Classification

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Alpha-melanocyte-stimulating hormone (α-MSH) is a tridecapeptide derived from proopiomelanocortin that exhibits potent anti-inflammatory properties by regulating the production of inflammatory and mediators. This peptide has been well established in several inflammatory models, including inflammatory bowel disease (IBD). However, the extremely short duration in vivo limits its application in clinics. To address this limitation, Bifidobacterium is used as a carrier to deliver α-MSH. We utilised α-MSH-engineered Bifidobacterium against IBD, which is closely linked to immune and intestinal microbiota dysfunction. First, we constructed a Bifidobacterium secretion of α-MSH named B. longum-α-MSH. We then tested the recombinant α-MSH expression and determined its bioactivity in HT-29 cells. To assess its effectiveness, the engineered Bifidobacterium was used against an ulcerative colitis (UC) model of rats induced by dextran sulphate sodium. Data showed that α-MSH expression in B. longum-α-MSH was effective, and its biological activity was similar to the synthesized one. This UC model experiment indicated that B. longum-α-MSH successfully colonized the intestinal gut, expressed bioactive α-MSH and had a significant anti-inflammatory effect. Results demonstrated the feasibility of preventing IBD by using B. longum-α-MSH.
    Journal of Medical Microbiology 11/2015; DOI:10.1099/jmm.0.000197
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    ABSTRACT: We report on a molecular investigation carried out to ascertain the prevalence of drug resistant TB and the specific gene mutations responsible for resistance to rifampicin (RIF) and/or isoniazid (INH) in Iraq. One hundred and ten clinical isolates from category II TB cases from Baghdad (58%) and several Iraqi provinces (42%) were analyzed using colorimetric, low-cost and density (LCD) microarrays (MYCODirect and MYCOResist LCD-array kits, Chipron GmbH, Germany) to identify the point mutations responsible for resistance in Mycobacterium tuberculosis isolates. Seventy-six patients (69.1%) had resistant strains, of which 40 (36%) were MDRTB. Where mono-resistance was identified, it was found to be predominantly to RIF (83%). The most common mutations were rpoB S531L (50%), inhA C15T (25%), and katG S315T (15%). The most common MDRTB genotypes were rpoB S531L with inhA C15T (60%) and rpoB S531L with katG S315T (20%). Where phenotypic analysis of clinical isolates was also performed, genotypic data were found to show excellent correlation with phenotypic results. Correlation was found between the MYCOResist LCD-array and GenoType MTBDRplus for detection of resistance to RIF. Our study shows MDRTB in 36% of category II TB cases in Baghdad and surrounding Iraqi provinces which reflects World Health Organization (WHO) findings based on phenotypic studies. Diagnosis of TB and MDR-TB using culture-based tests are a significant impediment to global TB control. The LCD arrays investigated herein are easy to use, sensitive and specific molecular tools for TB resistance profiling in resource-limited laboratory settings.
    Journal of Medical Microbiology 11/2015; DOI:10.1099/jmm.0.000203
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    ABSTRACT: Clostridium difficile is the most frequently identified enteric pathogen in patients with nosocomial antibiotic-associated diarrhoea and pseudomembranous colitis. Several clinically isolated C. difficile strains are resistant to antibiotics other than metronidazole and vancomycin. Recently, bacteriocins of lactic acid bacteria have been proposed as an alternative or complementary treatment. The aim of this study was to investigate the inhibitory effect of nisin, a bacteriocin produced by several strains of Lactococcus lactis, against clinical isolates of C. difficile. Nisin Z obtained from culture of L. lactis subsp. lactis biovar. diacetylactis was tested along with commercial nisin A. The effect of nisin A on C. difficile spores was also examined. Nisin A and Z both inhibited the growth of all C. difficile isolates and the MIC was estimated at 6.2 µg.mL-1 for nisin Z and 0.8 µg.mL-1 for nisin A. Besides, C. difficile spores were also susceptible to nisin A (25.6 µg.mL-1) which dropped by 40-50 % spore viability. These results suggest that nisin and hence nisin-producing Lactococcus strains could be used to treat C. difficile-associated diarrhoea.
    Journal of Medical Microbiology 11/2015; DOI:10.1099/jmm.0.000202

  • Journal of Medical Microbiology 10/2015; DOI:10.1099/jmm.0.000178
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    ABSTRACT: Identification of bacteria causing lower airway infections is important to determine appropriate antimicrobial therapy. Flexible bronchoscopy with bronchoalveolar lavage (BAL) is used to obtain lower airway specimens in young children. The first lavage (lavage-1) is typically used for bacterial culture. However, no studies in children have compared the detection of cultivable bacteria from sequential lavages of the same lobe. BAL fluid was collected from two sequential lavages of the same lobe in 79 children enrolled in our prospective studies of chronic cough. The respiratory bacteria Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and H. parainfluenzae were isolated and identified using standard published methods. H. influenzae was differentiated from H. haemolyticus using PCR assays. Lower airway infection was defined as ≥104 cfu/mL BAL fluid. We compared cultivable bacteria from lavage-1 to the second lavage (lavage-2) using the kappa statistic. Lower airway infections by any pathogen were detected in 46% of first lavages and 39% of second lavages. Detection was similar in both lavages for all pathogens; the kappa statistic was 0.7-0.8 for all bacteria except H. parainfluenzae. Of all infections detected in either lavage, 90% were detected in lavage-1 and 78% in lavage-2. However, culture of lavage-2 identified infections that would have been missed in 8% of children, including infections by additional S. pneumoniae serotypes.Our findings support the continued use of lavage-1 for bacterial culture; however, culture of lavage-2 may yield additional identifications of bacterial pathogens in lower airway infections.
    Journal of Medical Microbiology 09/2015; DOI:10.1099/jmm.0.000173

  • Journal of Medical Microbiology 09/2015; DOI:10.1099/jmm.0.000166
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    ABSTRACT: Some species of the Mycobacterium tuberculosis complex (MTBC), particularly Mycobacterium tuberculosis that causes human tuberculosis (TB), are the first cause of death linked to a single pathogen worldwide. In the last decades, evolutionary studies have much improved our knowledge on MTBC history and have highlighted its long co-evolution with humans. Its ability to remain latent in humans, the extraordinary proportion of asymptomatic carriers (1/3 of the entire human population), the deadly epidemics and the observed increasing level of resistance to antibiotics are proofs of its evolutionary success. Many MTBC molecular signatures show that these bacteria are not only a model of adaptation to humans, but that they have also influenced human evolution. Due to the unbalance between the number of asymptomatic carriers and the number of patients with active TB, some authors suggest that infection by MTBC could have a protective role against active TB disease and also against other pathologies. However, it would be inappropriate to consider these infectious pathogens as commensals or symbionts, given the level of morbidity and mortality caused by TB.
    Journal of Medical Microbiology 09/2015; DOI:10.1099/jmm.0.000171
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    ABSTRACT: Tuberculosis (TB), an infectious disease that is caused by Mycobacterium tuberculosis complex (MTC), remains one of the leading causes of death in the world. In Korea, the current prevalence of multi-drug-resistant TB (MDR-TB) poses a major problem. The most common method for diagnosing TB in developing countries is sputum smear microscopy; however the sensitivity of this test is relatively low, and it usually requires well-trained laboratory staff. Cultures of MTC require up to several weeks in sophisticated facilities, such as Bio Safety Level-3. Effective diagnostic techniques are necessary to control TB. In Korea, we evaluated a loop-mediated isothermal amplification assay (LAMP) targeting the hspX gene (TB-hspX-LAMP) of MTC. For clinical evaluation, culture confirmation, smear microscopy, and TB-hspX-LAMP were performed on 303 sputum specimens obtained from suspected TB patients in Korea. The sensitivity, specificity, positive predictive value, and negative predictive value of TB-hspX-LAMP were 71.1, 98.8, 91.4, and 95.1 %, respectively, compared with TB culture, which is the gold standard for diagnosis of TB. In contrast, the comparable values of the smear microscopy were 24.4, 98.1, 68.8, and 88.2 %, respectively. Therefore, we concluded that the TB-hspX-LAMP was superior to the use of smear microscopy for the detection of MTC in sputum specimens in clinical settings in Korea.
    Journal of Medical Microbiology 09/2015; DOI:10.1099/jmm.0.000164

  • Journal of Medical Microbiology 09/2015; 64(9):1074-1081. DOI:10.1099/jmm.0.000110
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    ABSTRACT: A loop-mediated isothermal amplification (LAMP) assay was developed for the first time to detect Enterocytozoon bieneusi DNA from human faecal specimens. Four primers specific for E. bieneusi were designed corresponding to SSU rRNA gene sequences and tested on 100 human faecal specimens. Thirty-nine out of 100 faecal specimens (39%) were confirmed positive for E.bieneusi by LAMP as compared to polymerase chain reaction (PCR) (33/100; 33%) and light microscopy (32/100; 32%). LAMP yielded 94% sensitivity and 88% specificity compared to microscopy (sensitivity = 48%, specificity = 76%). No significant differences in positive detection of E. bieneusi were found among the three methods (p-value > 0.05). However, LAMP has showed a substantial agreement with PCR (κ = 0.78) and fair agreement was demonstrated between microscopy and PCR (κ = 0.25). In conclusion, the LAMP assay showed to be useful as a simplified, rapid, sensitive and specific alternative molecular screening tool in the diagnosis of E. bieneusi in faecal specimens.
    Journal of Medical Microbiology 08/2015; DOI:10.1099/jmm.0.000156
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    ABSTRACT: Listeria monocytogenes is a dangerous bacterium that causes the food-borne disease listeriosis and accounts for nearly 20% of food-borne deaths. This organism can survive the body's natural defenses within the digestive tract, including acidic conditions and bile. Though the bile response has been analyzed, limited information is available concerning the ability of L. monocytogenes to resist bile under anaerobic conditions, especially at acidic pH, which mimics conditions within the duodenum. Additionally, it is not known how the bile response varies between serotypes. In this study the survival of strains representing six serotypes were analyzed under aerobic and anaerobic conditions following exposure to bile. Exposure to bile salts at acidic pH increased toxicity of bile, resulting in a significant reduction in survival for all strains tested. However, following this initial reduction, no significant reduction was observed for an additional two hours except for 10403S (P = 0.002). Anaerobic cultivation increased bile resistance, but a significant increase was only observed in virulent strains when exposed to bile at pH of 5.5. Exposure to pH 3.0 prior to bile decreased viability among avirulent strains in bile at acidic conditions; oxygen availability did not influence viability. Together these data suggest that being able to sense and respond to oxygen availability may influence the expression of stress response mechanisms and that this response may correspond to disease outcome. Further research is needed on additional strains to determine how L. monocytogenes sense and respond to oxygen and how this varies between invasive and non-invasive strains.
    Journal of Medical Microbiology 08/2015; DOI:10.1099/jmm.0.000160

  • Journal of Medical Microbiology 08/2015; DOI:10.1099/jmm.0.000161
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    ABSTRACT: Salmonella Typhimurium is one of the leading serovars that causes salmonellosis worldwide. However, few studies have molecularly characterised S. Typhimurium strains in Brazil. In this study, we genotyped 92 S. Typhimurium strains isolated from humans(43) and food(49) between 1983-2013 in Brazil using PFGE, MLVA and ERIC-PCR. Moreover, we assessed the frequency of 12 virulence markers by PCR and the resistance profile against 12 antimicrobials. More than 85.8% of the strains studied carried 11 of the virulence markers or more. Thirty-three (25%) strains were multi-drug resistant (MDR). The 92 S. Typhimurium studied were grouped by PFGE in PFGE-A, PFGE-B1 and PFGE-B2; by MLVA in MLVA-A, MLVA-B1 and MLVA-B2 and, finally, were grouped by ERIC-PCR in ERIC-A and ERIC-B. The strains isolated from humans before the mid-1990s were allocated in all clusters. The strains isolated from humans after the mid-1990s were distributed in the PFGE-B1, MLVA-B1, MLVA-B2 and ERIC-A clusters. The strains isolated from food were distributed in all clusters, except in the PFGE-B2. All typing results suggest that the S. Typhimurium strains of human clinical origin isolated before the mid-1990s were genetically more diverse, which might indicate the selection of a more adapted S. Typhimurium subtype after S. Enteritidis became the most prevalent serovar in Brazil. Regarding strains isolated from food, the results suggest the current circulation of more than one subtype. Furthermore, the high frequency of virulence genes and the presence of MDR strains reinforces their potential hazard for humans and the risk of their presence in foods in Brazil.
    Journal of Medical Microbiology 08/2015; DOI:10.1099/jmm.0.000158

  • Journal of Medical Microbiology 08/2015; 64(10). DOI:10.1099/jmm.0.000157
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    ABSTRACT: The goal of this study was to compare biofilm synthesis among enterococci recovered from clinical samples (infection or colonization) of patients, as well as, environmental samples in order to determine possible virulence factors and clonal relationship. During a 2-year period, clinical samples (blood, catheter tips, bronchial secretions, wounds, peritoneal fluid, urine) and rectal swabs collected from hospitalized patients, as well as, environmental water samples were tested for the presence of Enterococcus faecalis and E. faecium. Antibiotic susceptibility testing was performed by the disk diffusion method and Etest. Strains were tested for the presence of vanA, vanB, esp, ace and asp genes by PCR. Clones were identified by PFGE (SmaI). From infected patients, 48 strains were identified; 24 E. faecium (10 vanA-positive, 14 vancomycin-susceptible) and 24 E. faecalis (one vanA-positive, 23 vancomycin-susceptible). Among 143 colonizing isolates, 134 were E. faecium (58 vanA-positive, 11 vanB-positive, 65 vancomycin-susceptible) and nine E. faecalis (three vanA-positive, two vanB-positive, four vancomycin-susceptible). Among 167 environmental water samples, 51 E. faecalis and 19 E. faecium, all glycopeptide-susceptible, were recovered. In total, 64 strains produced biofilm, whereas, 34 were esp-positive, 64 asp-positive, and 54 ace-positive. Biofilm production was associated with the presence of esp (P<0.001) and ace genes (P 0.021), being higher in infecting (P <0.001), and water (P 0.005) isolates as compared to colonizing ones. Clones of environmental water-strains were different than the patients' ones. The differences found in the incidence of antibiotic resistance, virulence factors and clones suggest that hospital and water enterococci are of different origin.
    Journal of Medical Microbiology 08/2015; DOI:10.1099/jmm.0.000151