Journal of Clinical Pathology (J Clin Pathol)

Publications in this journal

• Article: Histochemical detection of platelet esterase activity in the bone marrow postmortem: can megakaryocytes serve as indicators for time since death?

  Boehm J, Schmidt U, Veeck J, Porsche M, Schaefer HE
  Journal of Clinical Pathology 03/2013;
• Article: Frequent expression of fibroblast growth factor-23 (FGF23) mRNA in aneurysmal bone cysts and chondromyxoid fibromas.

  Graham R, Krishnamurthy S, Oliveira A, Inwards C, Folpe AL
  Journal of Clinical Pathology 10/2012;
• Article: Journal of Clinical Pathology

  Carr NJ
  Journal of Clinical Pathology 10/2012; 65(10):919.
• Article: Epigenomic profiling in polycythemia vera and essential thrombocythemia shows low levels of aberrant DNA methylation

  Barrio S, Gallardo M, Albizua E, Jimenez A, Rapado I, Ayala R, Gilsanz F, Martin-Subero JI, Martinez-Lopez J
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  ABSTRACT: AIMS: The purpose of this study was to compare the DNA-methylation signature in classic chronic Philadelphia negative myeloproliferative neoplasms (MPN), polycythaemia vera (PV) and essential thrombocythaemia (ET), in order to obtain a global insight into DNA-methylation changes associated with these malignancies. METHODS: Thirty-five MPN samples from 11 ET JAK2 V617F, 12 ET JAK2 wild type (WT) and 12 PV JAK2 V617F patients as well as 12 from healthy donors were analysed. DNA samples extracted from whole peripheral blood were hybridised to the "HumanMethylation27 DNA Analysis BeadChip." RESULTS: All groups showed a very homogeneous methylation pattern. Only the ZNF577 gene showed a differential methylation profile between PV JAK2 V617F positive and controls. This aberrant methylation was correlated with a differential gene expression of ZNF577. No aberrant hypermethylation was found in the SOCS-1 and SOCS-3 genes. CONCLUSIONS: According to our results, an aberrant methylation pattern does not seem to play a crucial role in MPN pathogenesis; nor does it justify phenotypical differences between PV and ET.
  Journal of Clinical Pathology 11/2011; 64(11):1010-3.
• Article: Vancomycin administration: the impact of multidisciplinary interventions.

  R K Crowley, F Fitzpatrick, D Solanki, S FitzGerald, H Humphreys, E G Smyth
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  ABSTRACT: The clinical microbiology team observed that patients were not receiving all prescribed doses of vancomycin. Ward staff was confused about ordering and interpreting vancomycin therapeutic drug monitoring (TDM) levels. To audit the incidence of vancomycin dose omission. To implement a series of interventions to improve vancomycin dose administration, and to repeat the audit process to assess these interventions. Three prospective audits were conducted to assess the impact of vancomycin TDM on administration of vancomycin. After the first audit, a number of changes in the TDM process were undertaken. After review of the second audit, a senior pharmacist coordinated ward-based pharmacists in assisting staff to interpret levels, and TDM interpretative charts were designed for drug charts. Following the third audit, feedback to hospital management and a plan for ongoing education were undertaken. There was a significant reduction in the number of vancomycin doses held inappropriately in the third (10% (78/782) of prescribed doses) when compared to the first audit (16% (161/1007) of doses) (p<0.01). Of doses that were held inappropriately, there was a significant decrease in doses held for no apparent reason in audit 3 (16% (27/170) of prescribed doses) when compared to audit 1 (25% (69/282) of doses) (p<0.05). The interventions resulted in a 37.5% reduction in inappropriately held vancomycin doses over a one-year period; 10% of doses are still being held inappropriately. This study highlights the difficulties in identifying barriers to change and changing healthcare worker behaviour.
  Journal of Clinical Pathology 11/2007; 60(10):1155-9.
• Article: Polyarteritis nodosa in hairy cell leukaemia: an autopsy report.

  Mahesha Vankalakunti, K Joshi, S Jain, R Nada, B D Radotra, S Varma
  Journal of Clinical Pathology 11/2007; 60(10):1181-2.
• Article: Fatal perimyocarditis in a migrant worker to the tropics from an unexpected aetiology: a case report.

  Azarisman Shah Mohd Shah, Mohd Daud Sulaiman, Noorfaizan Saidin, Oteh Maskon
  Journal of Clinical Pathology 11/2007; 60(10):1180-1.
• Article: Persistent parvovirus b19 infection resulting in carpal tunnel syndrome.

  Monica Musiani, Elisabetta Manaresi, Giorgio Gallinella, Marialuisa Zerbini
  Journal of Clinical Pathology 11/2007; 60(10):1177-8.
• Article: Hodgkin's lymphoma associated T-cells exhibit a transcription factor profile consistent with distinct lymphoid compartments.

  Cigdem Atayar, Anke van den Berg, Tjasso Blokzijl, Marcel Boot, Randy D Gascoyne, Lydia Visser, Sibrand Poppema
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  ABSTRACT: Hodgkin's lymphoma (HL) is characterised by an ineffective immune response that is predominantly mediated by CD4+ T-cells. To analyse the expression of the key regulatory T-cell transcription factors (TFs) in the T-cells of HL involved tissues in order to assess the nature of the T(H) immune response in HL. By immunohistochemistry, GATA3 was strongly and T-bet exclusively expressed in a subset of interfollicular lymphocytes in the reactive lymphoid tissues. In classical HL (CHL), which is generally located in the interfollicular zones, a predominance of T-bet+ T-cells and lesser amounts of GATA3+ and c-Maf+ T-cells was found, concordant with the pattern of the normal interfollicular compartment. In reactive lymphoid tissues, c-Maf was observed mostly in T-lymphocytes within the germinal centres (GCs). Nodular lymphocyte predominance type of Hodgkin's lymphoma (NLPHL) and progressively transformed germinal centres cases, showed a majority of c-Maf+ T-cells, consistent with the pattern in normal GCs. NLPHL cases uniformly showed c-Maf+/CD57+ T-cell rosettes around the neoplastic cells; these rosettes were absent in "paragranuloma-type" T-cell/histiocyte rich B-cell lymphoma. T-cell TF expression profiles of the reactive T-cells in both subtypes of HL are in accordance with the expression profile observed in the distinct lymphoid compartments.
  Journal of Clinical Pathology 11/2007; 60(10):1092-7.
• Article: Melanoma histopathology reporting: are we complying with the National Minimum Dataset?

  Manjit R Kaur, Peter S Colloby, Agustin Martin-Clavijo, Jerry R Marsden
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  ABSTRACT: The Royal College of Pathologists introduced the National Minimum Dataset (NMDS) for the histopathological reporting of cutaneous melanoma in February 2002. To determine if histological reporting of invasive primary cutaneous melanoma in the West Midlands region of the UK was compliant with the NMDS. Reports were identified from March 2002 to March 2003 via the regional Cancer Intelligence Unit, and compared with the NMDS. If all items of the NMDS were adhered to, the report was considered compliant. If not compliant, the report was checked to see if it included selected clinical and staging parameters. 543 cases of invasive cutaneous melanoma were identified, but only 407 reports were analysed. 69/407 (17%) (95% CI 14% to 20%) reports were fully compliant with the NMDS. Of the non-complaint reports, 45/361 (12%) (95% CI 9% to 16%) reported all staging and clinically relevant parameters; 62/361 (17%) (95% CI 59% to 65%) reported all staging parameters. Breslow thickness was reported in all but one of the reports (99.7%), Clark's level was reported in 344/407 (85%), ulceration in 280/407 (69%), and microsatellites in 146/407 (36%). There was slow uptake of the NMDS in this region in the year following its introduction. Although major parameters required for staging were more consistently reported, ulceration and microsatellites were less frequently reported.
  Journal of Clinical Pathology 11/2007; 60(10):1121-3.
• Article: Transfusion in sub-Saharan Africa: does a Western model fit?

  Stephen P Field, Jean-Pierre Allain
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  ABSTRACT: This review examines the current state of transfusion services in sub-Saharan Africa and presents the argument for and against the Western model of a centralised blood service with 100% voluntary non-remunerated blood donors as advocated by the World Health Organization. The current practice of family replacement donors in hospital-based blood service is the most economical option, but in the face of high child and maternal mortality rates the blood supply has proved to be insufficient. With estimates of 5-10% of HIV transmission in Africa being the result of contaminated blood transfusions, there is a need to improve testing for transfusion transmissible diseases and the selection of blood donors. Of major concern, with respect to testing, is the quality of kits being used and the continuity of supply. The need to produce components is discussed in the context of the transfusion needs in sub-Saharan Africa. The running costs of establishing and maintaining centralised blood services need careful consideration as such projects need to be sustainable in the future. It is concluded that both options are viable while centralised programmes are being developed, and a pragmatic approach should be taken to ensure that the patients' needs are met and that resources are suitably utilised to ensure sustainability.
  Journal of Clinical Pathology 11/2007; 60(10):1073-5.
• Article: Nodal aggressive B-cell lymphomas: a diagnostic approach.

  Sonam Prakash, Steven H Swerdlow
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  ABSTRACT: The diffuse aggressive B-cell lymphomas, as recognised in the 2001 WHO classification, represent a clinically and biologically heterogeneous group of neoplasms that require very different therapeutic approaches and have very different outcomes. They should be diagnosed using a multiparameter approach that emphasises morphological and immunophenotypic studies, and in at least some cases, relies on cytogenetic and/or genotypic studies. Incorporation of clinical data may be important as well. There is also current interest in going beyond the basic diagnosis and providing pathological prognostic information when possible. Whereas the diagnosis of some cases will be relatively easy, the differential diagnosis in others is very difficult, with some cases in a grey zone between two different well defined categories.
  Journal of Clinical Pathology 11/2007; 60(10):1076-85.
• Article: Cytophagic histiocytic panniculitis with fatal haemophagocytic lymphohistiocytosis in a paediatric patient with perforin gene mutation.

  Rong-Long Chen, Yung-Hsiang Hsu, Ikuyo Ueda, Shinsaku Imashuku, Kengo Takeuchi, Benjamin Pang-hsien Tu, Shih-Sung Chuang
  Journal of Clinical Pathology 11/2007; 60(10):1168-9.
• Article: Angiosarcoma of bone marrow with unusual expression of chymase: diagnosis in a trephine biopsy specimen.

  F Noack, L Balleisen, P Valent, H-P Horny
  Journal of Clinical Pathology 11/2007; 60(10):1183.
• Article: Carinal leiomyoma: a cause for acute respiratory distress.

  Ruta Goregaonkar, Pradeep Vaideeswar, Shobhana P Pandit
  Journal of Clinical Pathology 11/2007; 60(10):1183-4.
• Article: Hamazaki-Wesenberg bodies in two patients with no history of sarcoidosis.

  Ryan Winters, Masatoshi Kida, Kumarasen Cooper
  Journal of Clinical Pathology 11/2007; 60(10):1169-71.
• Article: An audit of UK pathologists' use of the British Society of Gastroenterology guidelines for the initial biopsy diagnosis of suspected chronic idiopathic inflammatory bowel disease.

  Roger M Feakins
  Journal of Clinical Pathology 11/2007; 60(10):1178-9.
• Article: Uterine tumour resembling ovarian sex cord tumour is an immunohistochemically polyphenotypic neoplasm which exhibits coexpression of epithelial, myoid and sex cord markers.

  D P Hurrell, W G McCluggage
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  ABSTRACT: To describe the clinicopathological and immunohistochemical findings in four cases of uterine tumour resembling ovarian sex cord tumour (UTROSCT). Four UTROSCTs were stained with a wide range of antibodies, including epithelial (AE1/3, epithelial membrane antigen), myoid (desmin, alpha smooth muscle actin, h-caldesmon), sex cord (alpha inhibin, calretinin, melan A, CD99) and neuroendocrine (chromogranin, CD56) markers as well as hormone receptors (oestrogen receptor, progesterone receptor, androgen receptor), vimentin, CD10, WT1 and HMB45. The tumours ranged from 0.8 to 19.5 cm. Three were relatively well circumscribed intramural myometrial lesions; the other was a pedunculated mass attached to the uterine serosa. The tumours were variably composed of solid, corded, trabecular, nested, glandular and retiform arrangements of tumour cells. In three cases, cells with eccentric nuclei and abundant eosinophilic cytoplasm, resulting in a rhabdoid appearance, were a prominent feature. Three cases were diffusely positive with AE1/3 and all with epithelial membrane antigen. Positivity with myoid markers was common with 3, 4 and 1 case respectively staining with desmin, alpha smooth muscle actin and h-caldesmon; 2, 4, 1 and 2 cases respectively were positive with alpha inhibin, calretinin, melan A and CD99. All were chromogranin negative and exhibited diffuse strong staining with CD56. All were diffusely positive with oestrogen receptor, progesterone receptor, vimentin and WT1. Three cases were androgen receptor positive and all were CD10 and HMB45 negative. UTROSCT exhibits a polyphenotypic immunophenotype with coexpression of markers of epithelial, myoid and sex cord lineage as well as hormone receptors.
  Journal of Clinical Pathology 11/2007; 60(10):1148-54.