International journal of systematic bacteriology (Int J Syst Bacteriol )

Publisher: International Union of Microbiological Societies; Society for General Microbiology; International Union of Microbiological Societies. Bacteria and Applied Microbiology Division; International Association of Microbiological Societies. International Committee on Bacteriological Nomenclature. Judicial Commission; International Association of Microbiological Societies. International Committee on Bacteriological Nomenclature; All authors

Description

Ijsb (International Journal of Systematic Bacteriology) has been discontinued. Now International Journal of Systematic and Evolutionary Microbiology.

  • Impact factor
    2.27
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    Impact factor
  • 5-year impact
    0.00
  • Cited half-life
    0.00
  • Immediacy index
    0.00
  • Eigenfactor
    0.00
  • Article influence
    0.00
  • Other titles
    International journal of systematic bacteriology, IJSB
  • ISSN
    0020-7713
  • OCLC
    1643282
  • Material type
    Periodical, Internet resource
  • Document type
    Journal / Magazine / Newspaper, Internet Resource

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Introduction. Campylobacter is one of the leading bacterial causes of food-borne disease. The prevalence of Campylobacter species resistant to antimicrobial agents is increasing. This study is intended to determine prevalence and antimicrobial susceptibility patterns of Campylobacter species among under-five children with diarrhea. Methodology. A cross-sectional study was conducted among 227 under-five children with diarrhea from July to October 2012 at Jimma town. Isolation and identification of Campylobacterspecies were performed using standard bacteriological techniques. Antimicrobial susceptibility test was performed following standard protocol. Chi-square and Fisher’s exact tests were used for analysis.Results. From227 under-five children, 16.7% were positive forCampylobacter spp.; isolates,C. jejuni,C. coli,andC. lari, accounted for 71.1%, 21.1%, and 7.9%, respectively. Higher rate of resistancewas observed to ampicillin76.3%, trimethoprim-sulfamethoxazole (68.4%), tetracycline (39.5%), chloramphenicol (31.6%), clindamycin (26.3%), and doxycycline (23.7%). Erythromycin, ciprofloxacin, gentamicin, norfloxacin, and nalidixic acid were effective formore than80%of the isolates.Multipledrug resistancewas observedamong 78.9%of all the three spp. Conclusions: Isolation rate of Campylobacter spp. was high.C. lari was reported for the first time at this study area.Higher rate of resistance was observed to the commonly used drugs.
    International journal of systematic bacteriology 01/2014; 2014(2014):1-7.
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    ABSTRACT: The ability of strains of faecal bacteria (Vibrio cholerae, Escherichia coli ATCC 25922, and four strains of Salmonella isolated, resp., from well water, pig, poultry, and human urine in Garoua) to survive or grow in well water microcosms was compared. Water samples were obtained from two wells in Garoua (north Cameroun). Autoclaving at 121°C for 15 min and filtration through 0.2 µm filter were used to make microcosms. Microcosms were constituted of unfiltered-autoclaved, filtered-nonautoclaved, and filtered-autoclaved well waters. Bacterial strains were inoculated at initial cell concentration of 3 Log10CFU/mL. All strains were able to survive/grow in used microcosms, and a maximal concentration of 5.61 Log10CFU/mL was observed. Survival abilities were strain and microcosm dependent. The declines were more pronounced in filtered-nonautoclaved water than in the other microcosms. E. coli and Salmonella sp. (poultry strain) lowered to undetectable levels (<1 Log10CFU/mL) after two days of water storage. V. cholera decreased over time, but surviving cells persisted for longer period in filtered-nonautoclaved water from well W1 (1.91 Log10CFU/mL) and well W2 (2.09 Log10CFU/mL). Competition for nutrients and/or thermolabile antimicrobial substances synthesized by “ultramicrocells” or by the autochthonous bacteria retained by the filter might affect the bacterial survival.
    International journal of systematic bacteriology 08/2013; 2013.
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    ABSTRACT: The objective of the present study was to describe the physiological properties of seven potential probiotic strains of Bacillus spp. Isolates were characterized morphologically, biochemically, and by 16S rRNA sequence analyses for identification. Tolerance to acidic pH, high osmotic concentrations of NaCl, and bile salts were tested. Isolates were also evaluated for their ability to metabolize different carbohydrates sources. The antimicrobial sensitivity profiles were determined. Inhibition of gastrointestinal Salmonella colonization in an avian model was also evaluated. Five strains of Bacillus were tolerant to acidic conditions (pH 2.0) and all strains were tolerant to a high osmotic pressure (NaCl at 6.5%). Moreover, all strains were able to tolerate concentration of 0.037% bile salts after 24 h of incubation. Three strains were able to significantly reduce Salmonella Typhimurium levels in the crop and in the ceca of broiler-type chickens. Among the 12 antibiotics tested for antibiotic resistance, all strains were resistant to bacitracin and susceptible to gentamycin, neomycin, ormethoprim, triple sulfa, and spectinomycin. Bacterial spore formers have been shown to prevent gastrointestinal diseases in animals and humans. The results obtained in this study show important characteristics to be evaluated when selecting Bacillus spp. candidates to be used as probiotics.
    International journal of systematic bacteriology 05/2013; 2013.
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    ABSTRACT: Activation of the complement component C3 is an important step in the complement cascade, contributing to inflammatory mechanisms. Considerable research on gene-disrupted mycobacterial strains using animal models of tuberculosis infection has reported the roles of some of the mycobacterial genes during tuberculosis infection. The aim of the present study was to assess the pattern of complement activation by the devR gene-disrupted Mycobacterium tuberculosis H37Rv strain and compare with that by its wild-type strain. In vitro complement activation at the level of C3 by the gene-disrupted strain, its complemented strain, and wild-type strain was performed using solid-phase ELISA. It was observed that the ability of devR gene-disrupted M. tuberculosis H37Rv to activate C3 was significantly reduced in comparison to its wild-type strain (). In addition, C3 activation by the complemented devR mutant strain was almost similar to that of the wild strain, which indicated that the reduced ability to activate C3 could potentially be due to the deletion of devR gene. These findings indicate that the gene devR probably aids in complement activation and contributes to the inflammatory processes during tuberculosis infection.
    International journal of systematic bacteriology 05/2013; 2013.
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    ABSTRACT: Pertussis or whooping cough is a highly infectious respiratory disease caused by Bordetella pertussis. In vaccinating countries, infants, adolescents, and adults are relevant patients groups. A total of 707 clinical specimens were received from major hospitals in Malaysia in year 2011. These specimens were cultured on Regan-Lowe charcoal agar and subjected to end-point PCR, which amplified the repetitive insertion sequence IS481 and pertussis toxin promoter gene. Out of these specimens, 275 were positive: 4 by culture only, 6 by both end-point PCR and culture, and 265 by end-point PCR only. The majority of the positive cases were from ≤3 months old patients (77.1%) (). There was no significant association between type of samples collected and end-point PCR results (). Our study showed that the end-point PCR technique was able to pick up more positive cases compared to culture method.
    International journal of systematic bacteriology 05/2013; 2013.
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    ABSTRACT: Objective. We compared laboratory developed real-time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M. hominis and Ureaplasma culture. Methods. 283 genitourinary specimens received in the clinical bacteriology laboratory for M. hominis and Ureaplasma species culture were evaluated. Nucleic acids were extracted using the Total Nucleic Acid Kit on the MagNA Pure 2.0. 5 μL of the extracts were combined with 15 μL of each of the two master mixes. Assays were performed on the LightCycler 480 II system. Culture was performed using routine methods. Results. M. hominis PCR detected 38/42 M. hominis culture-positive specimens, as well as 2 that were culture negative (sensitivity, 90.5%; specificity, 99.2%). Ureaplasma PCR detected 139/144 Ureaplasma culture-positive specimens, as well as 9 that were culture negative (sensitivity, 96.5%; specificity, 93.6%). Of the specimens that tested positive for Ureaplasma species, U. urealyticum alone was detected in 33, U. parvum alone in 109, and both in 6. Conclusion. The described PCR assays are rapid alternatives to culture for detection of M. hominis and Ureaplasma species, and, unlike culture, the Ureaplasma assay easily distinguishes U. urealyticum from parvum.
    International journal of systematic bacteriology 03/2013; 2013.
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    ABSTRACT: A thermophilic, anaerobic, spore-forming, dissimilatory Fe(III)-reducing bacterium, designated strain SR4T, was isolated from sediment of newly formed hydrothermal vents in the area of the eruption of Karymsky volcano on the Kamchatka peninsula. Cells of strain SR4T were straight-to-curved, peritrichous rods, 0.4-0.6 micron in diameter and 3.5-9.0 microns in length, and exhibited a slight tumbling motility. Strain SR4T formed round, refractile, heat-resistant endospores in terminally swollen sporangia. The temperature range for growth was 39-78 degrees C, with an optimum at 69-71 degrees C. The pH range for growth was 4.8-8.2, with an optimum at 6.3-6.5. Strain SR4T grew anaerobically with peptone as carbon source. Amorphous iron(III) oxide present in the medium stimulated the growth of strain SR4T; cell numbers increased with the concomitant accumulation of Fe(II). In the presence of Fe(III), strain SR4T grew on H2/CO2 and utilized molecular hydrogen. Strain SR4T reduced 9,10-anthraquinone-2,6-disulfonic acid, sulfite, thiosulfate, elemental sulfur and MnO2. Strain SR4T did not reduce nitrate or sulfate and was not capable of growth with O2. The fermentation products from glucose were ethanol, lactate, H2 and CO2. The G + C content of DNA was 32 mol%. 16S rDNA sequence analysis placed the organism in the genus Thermoanaerobacter. On the basis of physiological properties and phylogenetic analysis, it is proposed that strain SR4T (= DSM 12299T) should be assigned to a new species, Thermoanaerobacter siderophilus sp. nov.
    International journal of systematic bacteriology 11/1999; 49 Pt 4:1471-8.
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    ABSTRACT: Six strains of lactic acid bacteria isolated from sourdough were characterized taxonomically. They were Gram-positive, catalase-negative, facultatively anaerobic rods that did not produce gas from glucose. Morphological and physiological data indicated that the strains belong to the genus Lactobacillus and they were similar to Lactobacillus alimentarius in phenotypic characteristics. These strains shared the same phenotypic characteristics and exhibited intragroup DNA homology values of over 89.8%, indicating that they comprised a single species. The G + C content of the DNA for the strains was 37.2-38.0 mol%. The 16S rRNA sequence of representative strain TB 1T was determined and aligned with that of other Lactobacillus species. This strain was placed in the genus Lactobacillus on the basis of phylogenetic analysis. L. alimentarius was the most closely related species in the phylogenetic tree and this species also showed the highest sequence homology value (96%) with strain TB 1T. DNA-DNA hybridization indicated that strain TB 1T did not belong to L. alimentarius. It is proposed that these strains are placed in the genus Lactobacillus as a new species, Lactobacillus paralimentarius sp. nov. The type strain of L. paralimentarius is TB 1T, which has been deposited in the Japan Collection of Microorganisms (JCM) as strain JCM 10415T.
    International journal of systematic bacteriology 11/1999; 49 Pt 4:1451-5.
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    ABSTRACT: Re-evaluation of the taxonomic position of strain K-252T, which produces the compound K-252a, showed that the strain does not belong to the genus Nocardiopsis suggested previously. Strain K-252T formed aerial mycelia with long spore chains, and hyphal fragmentation was not observed. The cell wall chemotype of the strain was III/B, the major menaquinone was MK-9 (III, VIII-H4), the phospholipid pattern was PIV and the major cellular fatty acids were 10Me-C17:0, iso-C16:0 and C16:0. Phylogenetic analysis of the 16S rRNA sequence showed that strain K-252T was clustered in the Nonomuraea group. Furthermore, on the basis of DNA-DNA reassociation and phenotypic data, strain K-252T (= NRRL 15532T) was classified as a new species of the genus Nonomuraea. This strain is proposed as Nonomuraea longicatena sp. nov.
    International journal of systematic bacteriology 11/1999; 49 Pt 4:1623-30.

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