Acta biologica Cracoviensia. Series botanica (ACTA BIOL CRACOV BOT )
Acta Biologica Cracoviensia Series Botanica publishes original papers embodying the results of experimental or theoretical research, invited reviews, and brief communications in: Anatomy; Morphology; Cytology; Genetics; Karyology; Embryology; Tissue culture; Physiology; Biosystematics.
- Impact factor0.61Show impact factor historyHide impact factor history
- 5-year impact0.73
- Cited half-life7.10
- Immediacy index0.13
- Article influence0.17
- WebsiteActa Biologica Cracoviensia - Series Botanica website
- Other titlesActa biologica cracoviensia. Series. Botanica
- Material typePeriodical, Internet resource
- Document typeJournal / Magazine / Newspaper, Internet Resource
Publications in this journal
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ABSTRACT: Cryopreservation (i.e. storage at the temperature of liquid nitrogen; LN -196oC) is the most safe and cost-effective long-term conservation method of non-orthodox seed species. Somatic embryogenesis, on the other hand, is considered to be the most efficient (micro)propagation technique. By combining in vitro tissue culture techniques with cryoconservation it is possible to develop highly-diverse gene banks of both vegetatively and generatively propagated species on a small surface at reduced costs. The application of embryonic tissue for storage in LN is very beneficial, especially with endangered species, since it does not require injuring the mother plant (Lema-Rumińska and Kulus 2012). The seeds are very often stored at sub-zero temperatures. Their great advantage is the fact that they show a decrease in water content in comparison to vegetative tissues, which is the bottleneck for cryopreservation success. Overtime zygotic embryos or their axes of about 100 species, and somatic embryos of approximately 40 species of plants from different climates have been cryopreserved with variable survival and/or regrowth rates (Engelmann 2011). The cryopreservation procedures are developed better for the latter ones. For several species, an attempt of freezing embryogenic callus has been also made. This may be a good method for maintaining its embryonic potential. There are even some reports referring to embryogenic potential or metabolic activity growth of proembryogenic masses (PEMs) of some species observed after freezing. In the past various cryopreservation techniques have been applied. As for seeds direct immersion in liquid nitrogen, or simple air-drying (for 1-5 h) is possible. With some species these techniques can be even applied with embryos. Still, the so-called modern methods (e.g. preculture, vitrification, droplet-vitrification or their combinations) are usually more efficient. As for PEMs, the encapsulation-based techniques are the most often applied. There are also reports on employing slow-freezing for embryogenic tissues. All the protocols, however, need to be adjusted not only to the individual needs of species but also even to single cultivars. References Engelmann F. (2011). Cryopreservation of embryos: an overview. Methods in Molecular Biology 710: 155-154. Lema-Rumińska, J., Kulus, D. (2012). Induction of somatic embryogenesis in Astrophytum asterias (Zucc.) Lem. in the aspect of light conditions and auxin 2,4-D concentrations. Acta Scientiarum Polonorum – Hortortorum Cultus 11(4): 77-87.Acta biologica Cracoviensia. Series botanica 05/2014; 56(1):68.
- Acta biologica Cracoviensia. Series botanica 01/2014; 56(1):1-7.
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ABSTRACT: The genus Crataegus is a critical taxon. Since the mid-20th century the differentiation criteria and status of this particular species changed a few times. The overall hawthorn systematics of European hawthorns was created by Christensen (1992). In Poland there are six species, according to his approach.Acta biologica Cracoviensia. Series botanica 01/2013; 55(suppl. 1):61.
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