Departments View all

Department of Gastroenterology
Total Impact Points
Graduate School of Nanobioscience
Total Impact Points
Department of Urology
Total Impact Points

Publication History View all

  • [Show abstract] [Hide abstract]
    ABSTRACT: Background The visualization of induced pluripotent stem (iPS) cells with the use of fluorescent techniques is useful for the in vivo evaluation of iPS-derived functional cells following differentiation and distribution of the transplanted cells. The data obtained from the fluorescently labeled iPS cells would lead to amelioration and validation of protocols directing the differentiation of iPS cells into functional cells. In this study, we established enhanced green fluorescent protein (EGFP)–labeled iPS cells to enable their easy visualization. Methods Human iPS cells were transfected with (a) 2 transcription activator–like effector nuclease (TALEN) vectors targeted to the adeno-associated virus integration site 1 (AAVS1) locus and (b) the targeting vector carrying the homology arms, EGFP gene, and a drug-selection marker. Results Several puromycin-resistant clones were obtained after transfection of the targeting vector and corresponding TALEN-expressing vectors. EGFP expression in these clones was observed with the use of a fluorescent microscope. Clones were examined for specific recombination, which revealed precise targeting at the AAVS1 locus. Furthermore, EGFP protein expression was sustained after directed differentiation into a hepatic lineage. Conclusions We were successful in evaluating the behavior of iPS-derived hepatic cells. The data suggest that genomic knock-in at the AAVS1 locus is suitable for long-term observation of iPS-derived cells. Manipulation of the iPS genome can also be applied for the purification of hepatic cells during iPS cell differentiation by introducing the fluorescent protein under the regulation of a hepatic cell–specific promoter. Another application involves gene correction of iPS cells from patients with hepatic disease for regenerative medicine.
    Transplantation Proceedings 01/2014; 46(4):1205–1207.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Takotsubo cardiomyopathy (TC) is a recently recognized novel cardiac syndrome characterized by transient left ventricular dysfunction without obstructive coronary disease, electrocardiographic (ECG) changes (ST-segment elevation and/or negative T wave) or elevated cardiac enzymes. Because the clinical features and ECG findings of TC mimic those of anterior acute myocardial infarction (AMI) with occlusion of the left anterior descending coronary artery, differential diagnosis has an important role in selecting the most appropriate treatment strategy. Especially in the acute phase, differential diagnosis is essential for deciding whether reperfusion therapy is required. Although it has been suggested that ECG does not allow reliable differentiation between TC and anterior AMI, several ECG criteria distinguishing TC from anterior AMI have been proposed. In this review, we discuss ECG findings of TC, especially in the acute phase, compare them with those of anterior AMI, and identify ECG features that may facilitate early recognition of this disease.
    Journal of electrocardiology 01/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background context Rapid diagnosis and accurate detection of etiological agents in pyogenic spinal infection (PSI) patients are important. Purpose The purpose of this study was to evaluate the clinical usefulness of methicillin-resistant Staphylococcus-specific polymerase chain reaction (MRS-PCR) and broad-range universal PCR (U-PCR) for diagnosing PSI. Study design A prospective diagnostic study. Patients Thirty-two clinically suspect PSI patients and six control patients who underwent computerized tomography–guided biopsy and/or surgical treatment were enrolled. Methods Tissue samples were examined by microbiological culture, histopathology, and real-time PCR (MRS-PCR and U-PCR). The diagnostic accuracy of real-time PCR was analyzed based on the definitive diagnosis of infection, defined as a positive result from microbiological culture or histopathology. Results All six control subjects were negative for PSI for all analyses. Twelve clinically suspect PSI subjects received definitive diagnoses (PSI group). The non-PSI group consisted of six control subjects plus the remaining 20 patients from the PSI clinically suspect group. MRS-PCR results were positive for all MRS-cultured PSI subjects. U-PCR was positive for all subjects in the PSI group with one discrepancy between real-time PCR and microbiological culture results in differentiation between gram-positive and gram-negative bacteria. In the non-PSI group, MRS-PCR and U-PCR were positive in three and seven cases, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value of MRS-PCR for diagnosing MRS infection were 1.00, 0.91, 0.57, and 1.00, respectively; those for the diagnosis of bacterial infection with U-PCR were 1.00, 0.73, 0.63, and 1.00, respectively. Conclusion Identification of MRS infection and ability to differentiate between gram-positive and gram-negative bacteria is rapidly achieved using MRS-PCR and U-PCR. Real-time PCR provides a sensitive molecular diagnosis of PSI and may contribute to antibiotic selection.
    The Spine Journal. 01/2014; 14(2):255–262.


  • Address
    Yokohama-shi, Japan
  • Head of Institution
  • Website
Information provided on this web page is aggregated encyclopedic and bibliographical information relating to the named institution. Information provided is not approved by the institution itself. The institution’s logo (and/or other graphical identification, such as a coat of arms) is used only to identify the institution in a nominal way. Under certain jurisdictions it may be property of the institution.

202 Members View all

View all

Top publications last week by downloads

Journal of thoracic oncology: official publication of the International Association for the Study of Lung Cancer 01/2009; 3(12):1384-90.
Science 07/2014; 345(6194):1250092.

Top Collaborating Institutions


This map visualizes which other institutions researchers from Yokohama City University have collaborated with.

Rg score distribution

See how the RG Scores of researchers from Yokohama City University are distributed.