University of Santiago de Compostela

Santiago de Compostela, A Coruña, Spain

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    ABSTRACT: A new analytical procedure involving the use of water and a low percentage of ethanol combined to high temperature liquid chromatography–tandem mass spectrometry has been developed for the determination of nine high-intensity sweeteners in a variety of drink samples. The method permitted the analysis in 23 min (including column reequilibration) and consuming only 0.85 mL of a green organic solvent (ethanol). This methodology provided limits of detection (after 50-fold dilution) in the 0.05–10 mg/L range, with recoveries (obtained from five different types of beverages) being in the 86–110% range and relative standard deviation values lower than 12%. Finally, the method was applied to 25 different samples purchased in Spain, where acesulfame and sucralose were the most frequently detected analytes (>50% of the samples) and cyclamate was found over the legislation limit set by the European Union in a sample and at the regulation boundary in three others.
    Food Chemistry. 01/2015; 169:162–168.
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    ABSTRACT: New-born calves depend upon colostrum intake for the acquisition of immunoglobulins (Ig) and other beneficial substances. However, colostrum is also a source of reactive oxygen species (ROS). Intrinsic production of ROS also increases after birth, so the combination of colostral and intrinsic ROS could overwhelm the antioxidant capacity of the calf leading to oxidative stress (OS), a condition that has been shown to play a key role in the initiation and development of several pathological conditions. The aim of this observational study was to assess the effects of the redox balance of colostrum on the oxidative status of calves and on passive immune transfer. Serum samples were taken from 20 calves on their day of birth, every week during their first month of life and at 2 and 3 months of age, and the concentrations of ROS and serum antioxidant capacity (SAC) assayed. The oxidative/anti-oxidative profile and IgG content of the colostrum were also assessed. The redox balance of the colostrum had a significant effect on both calf oxidative status and on passive immune transfer (as measured by calf serum IgG concentration), which indicates that the oxidative/antioxidative profile of colostrum should be measured when colostrum quality is assessed. The highest risk of OS during the study period was found to be when the calves were fed artificial milk replacer; this suggests that calves should be supplemented with antioxidants during this period in order to minimize any harmful consequences of high ROS generation.
    The Veterinary Journal 12/2014;
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    ABSTRACT: The levels of arsenic (As) in the main commercial species of mushrooms present in Galicia, in their growth substrates, and mushroom supplements have been analysed by ICP-MS, with the intention of assessing potential health risks involved with their consumption. The mean concentrations of As in wild and cultivated mushrooms was 0.27 mg/kg dw, in mushroom supplements 0.40 mg/kg dw, in soils 5.10 mg/kg dw, and in growth substrate 0.51 mg/kg dw. No significant differences were observed between species, although the species Lactarius deliciosus possessed a slightly more elevated mean concentration (at 0.49 mg/kg dw) than the other species investigated. In soils, statistically significant differences (p <0.05) were observed according to geographic origin. Levels in mushroom supplements, although low, were higher than in wild or cultivated mushrooms. Measured arsenic levels were within the normal range in samples analysed in unpolluted areas. Because of the low As concentrations found in fungi and mushroom supplements from Galicia, and considering the relatively small inclusion of these foods in people's diet, it can be concluded that there is no toxicological risk of arsenic associated with the consumption of the species of mushrooms analysed or at the dosages indicated for mushroom supplements.
    Food and Chemical Toxicology. 08/2014; 73.

Information

  • Address
    Colexio de San Xerome, Praza do Obradoiro, s/n., 15782, Santiago de Compostela, A Coruña, Spain
  • Head of Institution
    Juan Viaño Rey
  • Website
    www.usc.es
  • Phone
    0034881 811 001
  • Fax
    0034881 811 201
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