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Faculty of Medicine
34
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17
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    ABSTRACT: A pot culture experiment was conducted in glass house at the International Atomic Energy Agency (IAEA) laboratories, Seibersdorf, Austria to select suitable drought tolerant wheat genotype using carbon isotopes discrimination technique. Four wheat genotypes viz., LU-26s, Bhittai, Roshan, Taifu, were tested. There were two treatments. i. Control (normal irrigation) and ii. Drought (@ 30 % field capacity of the control treatment). Drought treatment was imposed after two weeks of germination. The experiment was continued up to 10 weeks after germination and was terminated at flowering stage. The data was recorded in terms of plant height, number of tillers, shoot dry weight and carbon isotopic ratio (δ). Results showed that there was a significant decrease in all the growth parameters. The decrease was also significant among the genotypes. The genotypes LU-26s was found to have best performance under drought condition, with minimum decrease in the growth parameters i.e., plant height, number of tillers and shoot dry weight. High corbon isotopes discrimination (CID) values were also observed in genotype LU-26s, showing close positive correlation between SDW and CID. It is therefore concluded that carbon isotopes discrimination can be an important criterion for the selection of wheat genotypes for drought prone areas.
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    ABSTRACT: Leprosy (Hansen's disease) is a chronic granulomatous infection caused by Mycobacterium leprae with peripheral neuropathy as cutaneous and neurological manifestations. Peripheral nerve regeneration may be stimulated by vascular endothelial growth factor and other growth factors (GFs) that have important roles in extracellular matrix regeneration. All of those GFs can be found in platelet-rich plasma (PRP) preparation. The effect of PRP injection in leprosy peripheral neuropathy has never been reported. A double-blind, randomized, control clinical trial was conducted among 60 patients with leprosy peripheral neuropathy. They were randomized to receive either a 1-ml injection of PRP as treatment or a 1-ml injection of platelet-poor plasma (PPP) as control. Skin sensibilities were measured by two-point discrimination test (TPDT) and visual analog scale (VAS), which were taken before and two weeks after treatment. Perineural injection of PRP was shown to be significantly more effective than PPP (P < 0.05) either in VAS or TPDT measurements. In both groups, the patients had a tingling sensation at the time of injection that disappeared shortly after. This study shows that perineural PRP injection could promote improvement of peripheral neuropathy sensibility in patients with leprosy. More research is needed to better determine the effects of PRP in nerve regeneration.
    International journal of dermatology 10/2013;
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    ABSTRACT: Previously, we reported that minocycline, kanamycin and norfloxacin improved the survival rate in the E32511 model that we developed (Infect. Immun. 62: 3447-3453, 1994), but fosfomycin did not. In this study, we investigated the effectiveness of azithromycin (AZM) against Stx2d-producing EHEC O91:H21 strain B2F1 or Stx2c-producing Escherichia coli strain E32511 treated with Mitomycin C in vivo. Recently, we reported the effectiveness of AZM in our model and AZM strongly inhibited the release of Stx2c from E32511 in vitro (PLOS ONE e58959, 2013). However, it was very difficult to completely eliminate E32511 in the mouse feces by treatment with AZM alone. In this report, only AZM or Daio effectively promoted survival of mice infected with B2F1 compared to untreated mice. Furthermore, Daio inhibited the colonization of GFP-expressing B2F1 in the mouse intestine. Similarly, a combination of AZM and Daio in the E32511-infected mice reduced E32511 in the mouse feces and significantly improved survival.
    Microbial Pathogenesis 10/2013;

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