Osnabrück, Lower Saxony, Germany

Departments View all

Total Impact Points
Total Impact Points
Total Impact Points

Recent Publications View all

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Lettuce cultivars are not only amongst the most popular vegetables eaten raw, they are also involved in severe pathogen outbreaks world-wide. While outbreaks caused by Enterobacteriaceae species are well-studied, less is known about their occurrence in natural environments as well as the impact of biotic stress. Here, we studied the ecology of the human health-relevant bacterial family Enterobacteriaceae and assessed the impact of biotic disturbances by a soil-borne phytopathogenic fungus and Gastropoda on their structure and abundance in mesocosm and pot experiments. Using a polyphasic approach including network analyses of 16S rRNA gene amplicon libraries, quantitative PCR and complementary fluorescence in situ hybridization (FISH) microscopy we found substantial yet divergent Enterobacteriaceae communities. A similar spectrum of 14 genera was identified from rhizo- and phyllospheres but the abundance of Enterobacteriaceae was on average 3fold higher in phyllosphere samples. Both stress factors shifted the bacterial community of the leaf habitat, characterized by increases of species abundance and diversity. For the rhizosphere, we observed significant structural shifts of Enterobacteriaceae communities but also a high degree of resilience. These results could be confirmed by FISH microscopy but it was difficult to visualize phyllosphere communities. Additional inoculation experiments with Escherichia coli as model revealed their presence below the wax layer as well as in the endosphere of leaves. The observed presence influenced by stress factors and the endophytic life style of Enterobacteriaceae on lettuce can be an important aspect in relation to human health.
    PLoS ONE 02/2015; 10(2):e0118068. DOI:10.1371/journal.pone.0118068
  • [Show abstract] [Hide abstract]
    ABSTRACT: Problems of loading, unloading and premarshalling of stacks as well as combinations thereof appear in several practical applications, e.g. container terminals, container ship stowage planning, tram depots or steel industry. Although these problems seem to be different at first sight, they hold plenty of similarities. To precisely unite all aspects, we suggest a classification scheme and show how problems existing in literature can be described with it. Furthermore, we give an overview of known complexity results and solution approaches.
    European Journal of Operational Research 12/2014; 239(2):297–312. DOI:10.1016/j.ejor.2014.03.011
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The intracellular pathogen Salmonella enterica serovar Typhimurium causes intestinal inflammation characterized by edema, neutrophil influx and increased pro-inflammatory cytokine expression. A major bacterial factor inducing proinflammatory host responses is lipopolysaccharide (LPS). S. Typhimurium ΔmsbB possesses a modified lipid A, has reduced virulence in mice, and is being considered as a potential anti-cancer vaccine strain. The lack of a late myristoyl transferase, encoded by MsbB leads to attenuated TLR4 stimulation. However, whether other host receptor pathways are also altered remains unclear. Nod1 and Nod2 are cytosolic pattern recognition receptors recognizing bacterial peptidoglycan. They play important roles in the host's immune response to enteric pathogens and in immune homeostasis. Here, we investigated how deletion of msbB affects Salmonella's interaction with Nod1 and Nod2. S. Typhimurium ΔmsbB-induced inflammation was significantly exacerbated in Nod2-/- mice compared to C57Bl/6 mice. In addition, S. Typhimurium ΔmsbB maintained robust intestinal colonization in Nod2-/- mice from day 2 to day 7 p.i., whereas colonization levels significantly decreased in C57Bl/6 mice during this time. Similarly, infection of Nod1-/- and Nod1/Nod2 double-knockout mice revealed that both Nod1 and Nod2 play a protective role in S. Typhimurium ΔmsbB-induced colitis. To elucidate why S. Typhimurium ΔmsbB, but not wild-type S. Typhimurium, induced an exacerbated inflammatory response in Nod2-/- mice, we used HEK293 cells which were transiently transfected with pathogen recognition receptors.
    PLoS ONE 11/2014; 9(11):e113645. DOI:10.1371/journal.pone.0113645


  • Address
    Seminarstrasse, 49069, Osnabrück, Lower Saxony, Germany
  • Website
Information provided on this web page is aggregated encyclopedic and bibliographical information relating to the named institution. Information provided is not approved by the institution itself. The institution’s logo (and/or other graphical identification, such as a coat of arms) is used only to identify the institution in a nominal way. Under certain jurisdictions it may be property of the institution.

876 Members View all

View all

Top publications last week by reads

Neuron 11/2015; 88(4):735-748. DOI:10.1016/j.neuron.2015.10.012
47 Reads
Global Environmental Change 08/2009; 19(3-19):354-365. DOI:10.1016/j.gloenvcha.2009.06.001
43 Reads

Top Collaborating Institutions


This map visualizes which other institutions researchers from Universität Osnabrück have collaborated with.

Rg score distribution

See how the RG Scores of researchers from Universität Osnabrück are distributed.