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Department of Veterinary Medicine
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    ABSTRACT: We have previously demonstrated that quercetin (Quer), a polyphenol widely found in vegetables, decreased glioma cell growth in vitro. Here, we asked whether this compound could affect glioma growth in an in vivo rat glioma model. We found that daily intraperitoneal Quer (50 mg/kg) injections lead to a concentration of 0.15 μg of Quer per gram of brain tissue, which increased the tumor volume in a time dependent manner. We observed a small reduction in lymphocytic infiltration, a marker of good prognosis in gliomas that was accompanied by a small reduction in cell viability of peripheral T-cells. Moreover, after Quer treatment neither body weight alteration nor liver pathology markers were detected. Although in vitro studies and massive literature reports point to the antitumoral properties of Quer, the present results indicate that great caution has to be taken in the design of clinical trials and the indiscriminate use of this polyphenol as dietary supplement.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 11/2013;
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    ABSTRACT: Persea cordata Mez. (Lauraceae) is a medicinal plant used in veterinary ethnopharmacology, which is a popular medicine used as an anti-inflammatory and healing agent, mainly on animal skin diseases, characterized by cutaneous open wounds, in South Brazil. The purpose of this study was to investigate a possible antiedematogenic effect of ethyl acetate (EtAc) and butanol (BuOH) polar fractions of P. cordata on Evans blue dye leakage induced by pro-inflammatory agents in rat skin. Male Wistar rats (180-200g, n=5-6) were pretreated with a single intraperitoneal administration of EtAc or BuOH (1 to 600mgkg(-1)) fractions followed by intravenous Evans blue dye injection (1%, 30mgkg(-1), i.v.), 60minutes before the injection of phlogistic agents. Animals received intradermal injections (0.05ml) of carrageenan (CAR, 300µg/site), 48/80 compound (C4880, 10µg/site), histamine (HIS, 0.3µg/site), serotonin (5-HT, 0.01µg/site), dextran (DEX, 200µg/site), bradykinin (BK, 0.003µg/site), capsaicin (CPS, 400µg/site), substance P (SP, 0.003µg/site) or prostaglandin E2 (PGE2 10nmol/site) and they were submitted to euthanasia after 60min. Skin samples were obtained in the extravasation sites of Evans blue dye. Skin fragments were soaked in formamide at 37°C (during 24h) for Evans blue extraction. The amount of dye leakage in the tissue fragment was determined by a spectrophotometer (620nm). In a very similar manner in terms of potency and efficacy, systemic administration of EtAc and BuOH fractions caused dose-dependent inhibition of vascular Evans blue dye leakage induced by phlogistic agents in the rat skin. The results obtained (ID50 values in mg.kg(-1) and maximal inhibition in %) with EtAc fraction, as follows were: CAR (34.42 and 63.0), 4880 (8.52 and 59.1), HIS (21.22 and 66.8), 5-HT (32.99 and 73.4), DEX (41.74 and 67.0), BK (34.03 and 68.0), CPS (100.7 and 77), SP (2.1 and 78.9) and PGE2 (133 and 71.0). BuOH fraction significantly inhibited CAR (25.9 and 70)-, 4880 (36.8 and 66)-, HIS (17.6 and 77)-, 5-HT (32.8 and 56)-, DEX (89.6 and 75)-, BK (28.0 and 66)-, CPS (136.37 and 71)-, SP (5.6 and 78)- and PGE2 (109.64 and 56)-induced VE, respectively. Systemic administration of P. cordata polar fractions exerts a non-specific inhibitory effect on microvascular leakage induced by pro-inflammatory agents in rat skin, probably to interfering with different biological systems involved in the development of the inflammatory process, reinforcing the popular use of P. cordata as an anti-inflammatory and healing agent for skin.
    Journal of ethnopharmacology 08/2013;
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    ABSTRACT: Abstract Keratinolytic proteases are microbial enzymes that hydrolyze keratins, recalcitrant proteins found in the epidermis and epidermal appendages of vertebrates. Keratin-rich materials are abundantly generated as wastes from agroindustrial activities, particularly the meat and poultry industries. Therefore, these enzymes are postulated for several applications, including the bioconversion of keratin-rich materials, utilization as animal feed supplements, action as de-hairing agents in tannery, prion degradation, among others. Consequently, production of keratinases in sufficient amounts is necessary to meet industrial and commercial demands. This manuscript presents an overview regarding the sources of keratinases, emphasizing general aspects and mechanisms, such as substrate induction and catabolite repression, controlling keratinase synthesis by Bacillus species. The current research status and main approaches employed to obtain keratinases are discussed in a biotechnological perspective, highlighting the role of keratin-rich wastes as growth substrates, the use of mathematical models to optimize keratinase yield and also investigations on the heterologous expression of keratinases.
    Critical Reviews in Biotechnology 08/2013;

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    Chapecó, Brazil
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