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    ABSTRACT: In this study, crude oil was extracted by heating and filtering of the residual fat obtained from the processing of mechanically separated meats of Nile tilapia (Oreochromis niloticus) and hybrid sorubim (Pseudoplatystoma corruscans × P. fasciatum) for protein concentrate obtaining. The crude oil was refined by the following steps: degumming with 85% phosphoric acid, neutralization with 20% NaOH, washing with hot water at 85 °C, drying at 90 °C, clarification with activated charcoal and filtration with diatomaceous earth and anhydrous sodium sulfate. The quality of crude and refined oils was verified by acidity, peroxide, iodine, refractive, and saponification indexes, beyond moisture, lipid and free fatty acids contents, and density. The results show that the refining reduced the acidity index of the crude oil, however, favored the oil oxidation, as demonstrated by the increase in peroxide index. In most of the cases there was no change in the identity of the fish oils.
    Food Chemistry 08/2014; 157:100–104. DOI:10.1016/j.foodchem.2014.01.121
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    ABSTRACT: Resumo -A produção de indivíduos haploides e posterior duplicação para obter os duplo-haploides em milho têm despertado grande interesse da comunidade científica. A técnica pode reduzir o tempo e o custo de obtenção de linhagens melhorando a eficiência na produção de novas cultivares híbridas. Ao que tudo indica a tecnologia é dominada pelas multinacionais do setor, mas para as pequenas empresas de sementes e no setor público ainda existem alguns entraves para torná-la viável a todos os programas de melhoramento. Essa revisão procura mostrar como ocorre a obtenção de duplo-haploides, as dificuldades encontradas nesse processo e os desafios a serem superados. palavras-chave: indutores de haploidia, duplo-haploide, duplicação cromossômica, Zea mays. doubled haploids: importance in the genetic breeding oF maiZe abstract -The production of haploid individuals and subsequent chromosome doubling to obtain double haploids (DH) in maize has created great interest in the scientific community. The technique can reduce the time and costs to obtain pure lines, and improve the efficiency on the production of new hybrid cultivars. Apparently the technique is dominated by multinational seed corporations, but for small seed companies and public sector there are still some obstacles to make it viable for all breeding program. This review attempts to show up how to obtain double haploids, the difficulties of the process and the challenges to be overcome.
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    ABSTRACT: Piper amalago is used in Brazilian folk medicine as diuretic and for the treatment of urinary calculus disease, although no scientific data have been described to support these effects. Thus, this study aims to evaluate the diuretic effects and antilithiatic activity of the ethanolic extract of P. amalago (EEPam). Ethanolic extracts of P. amalago (125, 250 and 500mg/kg) were orally administered in male Wistar rats (n=5) and urinary excretion was measured at intervals of up to 24h after administration. The antilithiasic effect of EEPam on calcium oxalate urolithiasis crystallization was examined in a turbidimetric model. The oral administration of all doses of EEPam significantly increased urine output after 24h when compared to control group. Moreover, the application of EEPam, induced an inhibitory effect on calcium oxalate crystallization. According to results, P. amalago extracts showed diuretic and natriuretic activity and antilithiasic effects.
    Phytomedicine: international journal of phytotherapy and phytopharmacology 11/2013; DOI:10.1016/j.phymed.2013.10.014


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    Dourados, Brazil
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