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Conference Paper: Age is Only a Number? Time in the Emergency DepartmentRoyal College of Emergency Medicine - Annual Scientific Conference (RCEM 2015), Manchester; 09/2015
Conference Paper: Seasons in the Emergency DepartmentRoyal College of Emergency Medicine - Annual Scientific Conference (RCEM 2015), Manchester; 09/2015
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ABSTRACT: RESOLFT super-resolution microscopy allows subdiffraction resolution imaging of living cells using low intensities of light. It relies on the light-driven switching of reversible switch-able fluorescent proteins (RSFPs). So far, RESOLFT imaging was restricted to living cells, because chemical fixation typically affects the switching characteristics of RSFPs. In this study we created a fusion construct (FLASR) consisting of the RSFP rsEGFP2 and the diva-lent form of the antibody binding Z domain from protein A. FLASR can be used analogous to secondary antibodies in conventional immunochemistry, facilitating simple and robust sample preparation. We demonstrate RESOLFT super-resolution microscopy on chemically fixed mammalian cells. The approach may be extended to other super-resolution approaches requiring fluorescent proteins in an aqueous environment.PLoS ONE 09/2015; 10(9). DOI:10.1371/journal.pone.0136233
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