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    ABSTRACT: Introduction Rapid diagnosis of bacterial infection is an important for patient management and appropriate therapy during the early phase of bacteria-induced disease. Among the existing techniques for identifying pathogens were less sensitive and time-consuming processes. PCR has the benefits of excellent sensitivity, resolution and reproducibility. Materials and methods A multiplex PCR assay was designed for simultaneous detection and diagnosis of C. perfringens, P. aeruginosa and K. pneumoniae in different clinical samples. A total of 46 clinical samples of patients suspected with the infections were obtained from Sri Venkateswara Institute of Medical Sciences, and used in the present study as test samples for detecting the pathogens. Results and conclusions Through this approach, the above pathogens were detected simultaneously with high specificity in pure cultures and from the blood and urine samples. The results were correlated with normal diagnostic process, and proved to be more sensitive and specific diagnostic technique in the simultaneous detection of C. perfringens, P. aeruginosa and K. pneumoniae.
    Pathogenesis 11/2015; 2(4):21-26. DOI:10.1016/j.pathog.2015.09.001
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    ABSTRACT: To know the impact of Piper betel leaf stalk (P. betel) extract on Protein and energy metabolism and its role in male albino rats.
    06/2015; 3(2). DOI:10.1016/S2305-0500(15)30002-6
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    ABSTRACT: Thin films of tungsten trioxide (WO3) have been prepared by RF reactive magnetron sputtering of tungsten target at different substrate temperatures in the range 303–673 K and at fixed oxygen partial pressure of 6×10−2 Pa and sputter pressure of 4 Pa. The effect of substrate temperature on the structural, morphological, optical and electrochromic properties of WO3 films was systematically studied. The films formed at 303 K were of X-ray amorphous, while those deposited at substrate temperatures ≥473 K were crystallized into orthorhombic phase WO3. The crystallite size of the films increased from 17 to 24 nm with increase of substrate temperature from 473 to 673 K. Raman studies confirmed that the presence of O–W–O and W=O bonds in WO3 films. The surface morphology of the films was significantly varied with substrate temperature. The optical transmittance data revealed that the optical band gap increased from 3.08 to 3.48 eV and refractive index increased from 2.18 to 2.26 with increase of substrate temperature from 303 to 673 K respectively. The WO3 films formed at substrate temperature of 473 K exhibited better optical transmittance modulation of 40% between colored and bleached state with a color efficiency of 33.8 cm2/C and diffusion coefficient of 1.85×10−11 cm2/s.
    Physica B Condensed Matter 12/2014; 454:141–147. DOI:10.1016/j.physb.2014.07.029


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Top publications last week by reads

Biotechnology and Applied Biochemistry 09/2014; 61(5). DOI:10.1002/bab.1209
113 Reads
Current Protein and Peptide Science 09/2007; 8(4):329-51. DOI:10.2174/138920307781369427
36 Reads

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