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ABSTRACT: In Oriental medicine, the state of blood stagnation is called "Oketsu," meaning preceding state or symptomatic of sickness. Acupuncture stimulation is often used clinically for the treatment of "Oketsu." The degree of "Oketsu" is indicated by tongue color and form, swelling, paroxysmal blushing, and dark circles under the eyes. The blood’s fluidity is generally thought to be one of the blood stagnation factor. "Oketsu" is now considered as physiological blood flow and is studied from the perspective of the blood’s fluidity and vascular resistance. In our preliminary research, acupuncture stimuli were very effective in treating conditions associated with a decrease in the fluidity of the blood, such as "Oketsu." In this review, we discuss recent progress in acupuncture therapy and reported mechanisms of its action; we then focus on our original findings on these topics. Furthermore, we propose new factors related to acupuncture stimuli, including the blood’s fluidity, and report our investigations, using the restraint stress method, on the mechanisms underlying acupuncture stimuli.Journal of Acupuncture and Meridian Studies 08/2014; 7(4). DOI:10.1016/j.jams.2014.04.008
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ABSTRACT: Purpose Proper occlusal contact is important for the long-term success of prosthodontic therapy. We clarified the effects of occlusal loading force on occlusal contact in natural dentition by comparing measured values for occlusal loading and occlusal contact area. Methods Masseter muscle activity was measured in 10 subjects (2 male, 8 female; mean age, 27 years) with natural dentition using electromyography, with clenching at full strength with nothing interposed between the upper and lower teeth defined as 100% maximum voluntary contraction (MVC). Pressure-sensitive film (Occluzer) was used to examine occlusal contact points at 20, 40, 60, 80, 100 and 120% MVC. A material for checking accuracy of fit (BiteEye) was used to examine occlusal contact points at 20, 40, 60 and 80% MVC. ANOVA and the Bonferroni method were used to assess the results, with the level of significance set at 5%. Coefficients of variation (CV) were also calculated by dividing the standard deviation by the mean. Results Occlusal loading and occlusal contact area increased with clenching strength; however, CV showed differences between the methods at low and high MVC. Conclusions With Occluzer, testing should be carried out at clenching strength ≥60% MVC. With BiteEye, testing should be carried out from light clenching strength at 20% MVC to moderate clenching strengths at 40–60% MVC. Occluzer and BiteEye (10 μm) gave similar occlusal contact areas at 60–80% MVC. These results suggest that combined use of Occluzer and BiteEye gives an accurate picture of occlusion from weak to strong clenching strength.Journal of Prosthodontic Research 08/2014; 59(2). DOI:10.1016/j.jpor.2014.07.001
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ABSTRACT: We developed a highly sensitive chemiluminescent (CL) assay for hydrogen peroxide using 10-methyl-9-(phenoxycarbonyl) acridinium fluorosulfonate (PMAC) that produced chemiluminescence under neutral conditions and applied it to an enzyme immunoassay (EIA). One picomole of hydrogen peroxide could be detected using the optimized PMAC-CL method and 6.2 × 10(-20) mol β-d-galactosidase (β-gal) could be detected by combining an indoxyl derivative substrate and the proposed PMAC-CL method. This highly sensitive CL β-gal assay was applied to an EIA for thyroid-stimulating hormone (TSH) using β-gal as a label enzyme; 0.02-100.0 μU/mL TSH in human serum could be assayed directly and with high reproducibility. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd.Luminescence 06/2014; 29(4). DOI:10.1002/bio.2555
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