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- SourceAvailable from: Yoshihito Maeda
- Review of World Economics 06/2015; DOI:10.1007/s10290-015-0223-z
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ABSTRACT: Selection of bone cells, particularly osteoblasts and osteocytes, for analysis of cellular processes and differentiation is a very important issue because bone remodeling is a highly complex and harmonized process, which includes molecular and cellular interactions and communications. In this study, we introduce a novel osteoblast and osteocyte selection method that uses atomic force microscopy and OB7.3, an antibody of Phex, which is a specific protein marker expressed on the surface of osteocytes. The elasticity and Phex expression levels were simultaneously detected by force spectroscopy using the OB7.3-modified atomic force microscopy probe on the bone cell surface. The elastic modulus was different between osteoblasts and osteocytes. Phex expression level was analyzed by the distribution of Phex-OB7.3 rupturing.Journal of Nanoscience and Nanotechnology 05/2015; 15(5). DOI:10.1166/jnn.2015.9536
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