Departments View all

Computational Genomics
12
Total Impact Points
7
Members

Publication History View all

  • [Show abstract] [Hide abstract]
    ABSTRACT: In this article we explore the epistemic and ontological relationship between science and law through the concept of individual in the Universal Declaration of the Human Genome and Human Rights. We argue for a better understanding of this relationship in order to foresee ethical and social consequences derived from Law adopting concepts with a strong scientific meaning.
    Revista de derecho y genoma humano = Law and the human genome review / Cátedra de Derecho y Genoma Humano/Fundación BBV-Diputación Foral de Bizkaia. 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: It has been suggested that the N-terminal strand of the light chain variable domain (VL) protects the molecule from aggregation by hindering spurious intermolecular contacts. We evaluated the impact of mutations in the N-terminal strand on the thermodynamic stability and kinetic of fibrillogenesis of the VL protein 6aJL2. Mutations in this strand destabilized the protein in a position-dependent manner, accelerating the fibrillogenesis by shortening the lag time; an effect that correlated with the extent of destabilization. In contrast, the effect on the kinetics of fibril elongation, as assessed in seeding experiments was of different nature, as it was not directly dependant on the degree of destabilization. This finding suggests different factors drive the nucleation-dependent and elongation phases of light chain fibrillogenesis. Finally, taking advantage of the dependence of the Trp fluorescence upon environment, four single Trp substitutions were made in the N-terminal strand, and changes in solvent exposure during aggregation were evaluated by acrylamide-quenching. The results suggest that the N-terminal strand is buried in the fibrillar state of 6aJL2 protein. This finding suggest a possible explanation for the modulating effect exerted by the mutations in this strand on the aggregation behavior of 6aJL2 protein.
    Biochemical and Biophysical Research Communications 12/2013;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Human parturition is associated with an intrauterine pro-inflammatory environment in the choriodecidua. Evidence that some mediators of this signaling cascade also elicit responses leading to labor prompted us to characterize the cellular sources of these mediators in the human choriodecidua. Leukocyte-enriched preparations from human choriodecidua (ChL) and intervillous placental blood leukocytes (PL) were maintained in culture. Secretions of inflammatory cytokines, chemokines, and MMP-9 were documented. Leukocyte phenotype of ChL and PL was determined by flow cytometry using specific fluorochrome-conjugated antibodies. ChL showed a distinct pro-inflammatory secretion pattern of cytokines and chemokines when compared with PL, including higher amounts of TNF-α and IL-6, and decreased secretions of IL-4 and IL-1ra. ChL also secreted more MIP-1α and MCP-1 and MMP-9 than PL. No significant differences were found in leukocytes subsets between compartments. Based on our findings, we propose that ChL isolated from fetal membranes at term are functionally different from PL and may collaborate to modulate the microenvironment linked to induction and progression of human labor.
    American Journal Of Reproductive Immunology 11/2013;

Information

  • Address
    Mexico City, Mexico
Information provided on this web page is aggregated encyclopedic and bibliographical information relating to the named institution. Information provided is not approved by the institution itself. The institution’s logo (and/or other graphical identification, such as a coat of arms) is used only to identify the institution in a nominal way. Under certain jurisdictions it may be property of the institution.

50 Members View all

View all

Top publications last week by downloads

 
Salud pública de México, ISSN 0036-3634, Vol. 50, Nº. 6, 2008, pags. 530-547. 01/2008;
205 Downloads
 
Advances in Protein Physical Chemistry 12/2008: chapter Advances and perspectives in protein engineering: From natural history to directed evolution of enzymes: pages 407-438; Tran...
14 Downloads

Top Collaborating Institutions

Collaborations

This map visualizes which other institutions researchers from Instituto Nacional de Medicina Genómica have collaborated with.

Rg score distribution

See how the RG Scores of researchers from Instituto Nacional de Medicina Genómica are distributed.