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    ABSTRACT: Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA(++)/DAPI(-) bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chromosomes 1 and 3 and interstitial in the short arm of chromosome 8. Among endive accessions, FISH procedures revealed conserved position and number of 5S and 45S rDNA sites (two and three pairs, respectively), associated with the CMA-positive bands. Notwithstanding, polymorphisms were detected within chicory accessions regarding the number and the distribution of rDNA sites in relation to the most frequent karyotype (two pairs with 45S and one with 5S rDNA). The karyological markers developed allowed karyotypic differentiation between both species, uncovering peculiarities in the number and position of rDNA sites, which suggest chromosome rearrangements, such as translocations in chicory cultivars. The interspecific and intraspecific polymorphisms observed emphasize the potential of karyomorphological evaluations, helping our understanding of the relationships and evolution of the group.
    Genetics and Molecular Biology 09/2013; 36(3):357-363. DOI:10.1590/S1415-47572013005000025
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    ABSTRACT: Line shape effects of the surface plasmon resonance (SPR) at the gold-water interface have been exploited, towards extending instrumental performance in the angular interrogation mode. Comprising a micro-fluidic set-up both, SPR-line broadening and associated signal symmetry/asymmetry features have been monitored in real time for selected adsorption systems and are compared with the simultaneously taken resonance angle that represents the commonly recorded SPR feature. Four angular broadening contributions have been identified: an intrinsic, temperature independent fraction, a temperature dependent part that dominates at ambient temperature, a surface morphology/roughness contribution and an adsorption related fraction. The latter, and substantially smaller contribution, is crucially affected by surface conditions, as the transition from hydrophobic to a hydrophilic surface state. Anionic sub-monolayer adsorption has been clearly resolved, along with detection of a pronounced calorimetric effect that originates from an exothermic surface reaction upon exposure of the protein covered gold surface to a strong chemical oxidant. KeywordsLine broadening-Signal symmetry/asymmetry-Surface plasmon resonance
    Plasmonics 09/2010; 5(3):259-266. DOI:10.1007/s11468-010-9140-0
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    ABSTRACT: RESUMO Este texto tem como objetivo abordar alguns aspectos da educação de jovens e adultos trabalhadores, como também as políticas educacionais, observando a educação e seus condicionantes estruturais na educação de jovens e adultos trabalhadores. Discutindo como as formas de organizações populares e sindicais ajudaram na construção do processo de transição democrática e na década de 80 surge o novo sindicalismo e demais movimentos sociais, que iniciam algumas propostas concretas da classe trabalhadora para erguer a bandeira de luta em defesa da escola pública, gratuita, universal e de qualidade. Palavras-chave: educação profissional, políticas educacionais, educação de jovens e adultos.


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Jornada de Atualização em Informática na Educação; 01/2012
V Colóqui Internacional Educação e Contemporaneidade; 09/2011

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