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    ABSTRACT: This study compared fish longevity, growth, and oxidative stress between normal and deformed Aphanius fasciatus collected from the Gulf of Gabes. For this purpose, fish were collected from control (S1) and polluted (S2) sites in Tunisian coast and percentages of spinal deformities were determined. Oxidative stress biomarkers were also compared between normal and deformed fish, including levels of protein sulfhydryl (SH), malondialdehyde (MDA), and metallothioneins (MT), as well as activities of superoxide dismutase (SOD) and alkaline phosphatase (AP). Levels of heavy metals (Cd, Cu, and Zn) and polycyclic aromatic hydrocarbons (PAH) were also measured. Data showed that the highest occurrence of deformities was observed in younger fish and decreased significantly thereafter with age. Chemical analysis demonstrated high levels of heavy metals and PAH at polluted sites compared to a reference location. In deformed fish, a growth perturbation marked by a decrease of growth rate and condition index (CI), a high accumulation of Cd was noted. In addition, oxidative stress marked by changes in the studied biomarkers was observed. Taken together, oxidative stress, diminished longevity, and a disturbance of growth may be considered to be responsible factors contributing to spinal deformities.
    Journal of Toxicology and Environmental Health Part A 01/2013; 76(23):1269-1281.
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    ABSTRACT: Gilthead sea bream larvae (Sparus aurata) aged 47 days post hatching (dph) (11.6-12.8 mg in wet weight) were exposed to several sublethal concentrations of Cd(2+) (0.1, 5 and 10 mg/L) during 6 days in order to investigate the effects of this heavy metal on the expression of selected genes involved in detoxification (metallothionein-mt, glutathione peroxidase 1-gpx1), stress response (heat shock protein 70-hsp70, tumour necrosis factor α-tnfα) and ossification (osteocalcin-oc) processes. For this purpose, specimens of 47 dph were exposed first for 72 h from 0.1 to 20 mg/L of Cd(2+) in order to evaluate the median lethal concentration (LC(50)) for this metal, which was determined at 15.32 mg/L. Considering the results regarding the relative transcript levels of gpx1 and hsp70, Cd(2+) at any of the tested levels (0.1, 5 and 10 mg/L) did not induce oxidative stress in gilthead sea bream larvae, whereas relative transcript levels of mt were increased at 5 and 10 mg/L of Cd(2+) probably to detoxify this metal excess. Relative transcript levels of tnfα were not level dependent and were down-regulated in larvae exposed to 5 and 10 mg/L of Cd(2+). At those concentrations, transcript levels of oc were down-regulated suggesting a disruption in bone mineralization. Results from this study provided insights in some molecular mechanisms underlying Cd(2+)-induced toxicity in fish at early stages of development. This is the first study to show that cadmium contamination can depress oc expression in teleosts.
    Fish Physiology and Biochemistry 10/2012;
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    ABSTRACT: Background  Reactive oxygen species play a key role in the development of many dermatological disorders. Objective  The purpose of this study is to examine the lipid peroxidation, protein oxidation and antioxidative profile in Tunisian pemphigus foliaceus (PF) patients. Methods  Malondialdehyde (MDA), conjugated dienes (CD), protein thiol levels, catalase (CAT) and superoxide dismutase (SOD) activities were evaluated in skin biopsies of 13 patients compared to biopsies of 7 healthy controls. Results  Oxidative stress was confirmed in these three types of patient biopsies as compared to controls. Thus, MDA, CD levels and catalase CAT and SOD activities were significantly increased in lesional, perilesional and normal biopsies of PF patients than in those of control subjects. Protein oxidative was confirmed by lower levels of protein thiols in lesional, perilesional and normal biopsies than in control's biopsies. Otherwise, in patients, a significant rise of these biomarkers was observed in lesional and perilesional biopsies compared with normal biopsies. Conclusion  This study shows that oxidative stress could be involved in the pathogenesis of PF by the spread of skin lesions and/or by the increase in auto-antibodies' reactivity.
    Journal of the European Academy of Dermatology and Venereology 06/2012;

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    Sfax, Tunisia
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Bioresource Technology 01/2008; 98(18):3535-46.
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Food science and biotechnology 01/2012; 21:1105-1112.
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